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    products/primary-antibodies/islet-1-antibody-ep4182-neural-stem-cell-marker-ab109517.pdf

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RecombinantRabMAb

Recombinant Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

  • Datasheet
  • SDS
Reviews (6) Submit a question References (40)

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Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
  • Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Chicken, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
    See all Islet 1 primary antibodies
  • Description

    Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
  • Host species

    Rabbit
  • Specificity

    86% identities with Islet 2
  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Chicken, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.
  • General notes

    Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.

    Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP4182
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cardiovascular
    • Atherosclerosis
    • Diabetes associated
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Transcription factors/regulators
    • Stem Cells
    • Neural Stem Cells
    • Neuron Restricted Lineage
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Pancreas development
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203404)
    • Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203405)
    • Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203406)
    • Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
    • Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] (ab275990)
    • Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab277925)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Islet 1 protein (ab134882)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/250.

For unpurified use at 1/50 - 1/100.

WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP
1/10 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
1/250.

For unpurified use at 1/50 - 1/100.

WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP
1/10 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds to one of the cis-acting domain of the insulin gene enhancer.
  • Tissue specificity

    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P61371 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3670 Human
    • Entrez Gene: 16392 Mouse
    • Omim: 600366 Human
    • SwissProt: P61371 Human
    • SwissProt: P61372 Mouse
    • Unigene: 505 Human
    • Unigene: 42242 Mouse
    • Alternative names

      • Insulin gene enhancer protein ISL 1 antibody
      • Insulin gene enhancer protein ISL-1 antibody
      • Insulin related protein antibody
      • ISL 1 antibody
      • ISL LIM homeobox 1 antibody
      • ISL1 antibody
      • ISL1 transcription factor LIM homeodomain antibody
      • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
      • ISL1 transcription factor, LIM/homeodomain antibody
      • ISL1_HUMAN antibody
      • Islet-1 antibody
      • Islet1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)

      Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.

    • Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

       

       

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

    • Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (40)

    Publishing research using ab109517? Please let us know so that we can cite the reference in this datasheet.

    ab109517 has been referenced in 40 publications.

    • Hosoya M  et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
    • Domínguez-Bautista JA  et al. Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol 9:587096 (2021). PubMed: 33575260
    • Li Y  et al. 4-phenylbutyrate exerts stage-specific effects on cardiac differentiation via HDAC inhibition. PLoS One 16:e0250267 (2021). PubMed: 33882103
    • Shi Q  et al. Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer. Mol Med 27:47 (2021). PubMed: 33962568
    • Huang X  et al. Human amyotrophic lateral sclerosis excitability phenotype screen: Target discovery and validation. Cell Rep 35:109224 (2021). PubMed: 34107252
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (anoderm)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    anoderm
    Blocking step
    anti-body diluent (BSA) as blocking agent for 30 minute(s) · Concentration: 00% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 17 2021

    IHC - Wholemount abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Zebrafish Embryo (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Enrico Mingardo

    Verified customer

    Submitted Nov 22 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Skin)
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Elena Ezhkova

    Verified customer

    Submitted Jan 22 2018

    ChIP abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    ChIP
    Sample
    Mouse Cell lysate - nuclear (Min6)
    Negative control
    Negative control for Isl-1 antibody: known locus without enrichment Positive control for IP: Antibody for another factor that is not enriched at that locus
    Specification
    Min6
    Detection step
    Real-time PCR
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1.1% Formaldehyed
    Positive control
    Positive control for Isl-1 antibody: known locus of enrichment Positive control for IP: Antibody for another factor also enriched at that locus
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 24 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Permeabilization
    Yes - saponin
    Specification
    Human pluripotent stem cell derived cardiomyocyte
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 16 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Chicken Tissue sections (hindbrain section)
    Permeabilization
    Yes - Triton-X
    Specification
    hindbrain section
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 17 2013

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