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RecombinantRabMAb

Recombinant Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Reviews (6) Submit a question References (52)
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
  • Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
  • Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
  • Reacts with: Mouse, Chicken, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free PE

Overview

  • Product name

    Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
    See all Islet 1 primary antibodies

  • Description

    Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker

  • Host species

    Rabbit

  • Specificity

    86% identities with Islet 2

  • Tested applications

    Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Chicken, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.

  • General notes

    Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.

    Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (1)
1/250.

For unpurified use at 1/50 - 1/100.
WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP
1/10 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/250.

For unpurified use at 1/50 - 1/100.
WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP
1/10 - 1/100.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds to one of the cis-acting domain of the insulin gene enhancer.

  • Tissue specificity

    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.

  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Insulin gene enhancer protein ISL 1 antibody
      • Insulin gene enhancer protein ISL-1 antibody
      • Insulin related protein antibody
      • ISL 1 antibody
      • ISL LIM homeobox 1 antibody
      • ISL1 antibody
      • ISL1 transcription factor LIM homeodomain antibody
      • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
      • ISL1 transcription factor, LIM/homeodomain antibody
      • ISL1_HUMAN antibody
      • Islet-1 antibody
      • Islet1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)

      Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
      Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 39 kDa

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.

    • Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control. 

       

       

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 39 kDa



      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

      ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

    • Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
      Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)

    References (52)

    Publishing research using ab109517? Please let us know so that we can cite the reference in this datasheet.

    ab109517 has been referenced in 52 publications.

    • Wang W  et al. The histone demethylase Kdm6b regulates subtype diversification of mouse spinal motor neurons during development. Nat Commun 13:958 (2022). PubMed: 35177643
    • Akter M  et al. Generation of highly pure motor neurons from human induced pluripotent stem cells. STAR Protoc 3:101223 (2022). PubMed: 35300000
    • Hosoya M  et al. Early development of the cochlea of the common marmoset, a non-human primate model. Neural Dev 17:6 (2022). PubMed: 35524278
    • Bauer US  et al. Validation of Functional Connectivity of Engineered Neuromuscular Junction With Recombinant Monosynaptic Pseudotyped ΔG-Rabies Virus Tracing. Front Integr Neurosci 16:855071 (2022). PubMed: 35669734
    • Kishimoto K  et al. Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems. Nat Protoc 17:2699-2719 (2022). PubMed: 35978039
    View all Publications for this product

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human pluripotent stem cell derived cardiomyocyte)
    Permeabilization
    Yes - saponin
    Specification
    Human pluripotent stem cell derived cardiomyocyte
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 16 2014

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