Recombinant Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-251148-anti-islet-1-antibody-ep4182-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker
- Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Chicken, Human
Related conjugates and formulations
Overview
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Product name
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker
See all Islet 1 primary antibodies -
Description
Rabbit monoclonal [EP4182] to Islet 1 - Neural Stem Cell Marker -
Host species
Rabbit -
Specificity
86% identities with Islet 2 -
Tested applications
Suitable for: ICC/IF, WB, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Chicken, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates. IHC-Fr: Chicken hindbrain tissue. ICC/IF: U-87 MG cell line.
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General notes
Insulin gene enhancer protein ISL-1 is a protein encoded by the ISL1 gene. Among other roles, ISL-1 plays a part in the embryogenesis of pancreatic islets of Langerhans and some models, deficiency have shown failure of neural tube motor neurons to differentiate.
Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP4182 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 594 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203404)
- Alexa Fluor® 555 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203405)
- Alexa Fluor® 568 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab203406)
- Anti-Islet 1 antibody [EP4182] - BSA and Azide free (ab216657)
- Alexa Fluor® 488 Anti-Islet 1 antibody [EP4182] (ab275990)
- Alexa Fluor® 647 Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab277925)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/250.
For unpurified use at 1/50 - 1/100. |
| WB |
1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
|
|
| IP |
1/10 - 1/100.
|
|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/250. For unpurified use at 1/50 - 1/100. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 39 kDa. |
|
IP
1/10 - 1/100. |
|
Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Binds to one of the cis-acting domain of the insulin gene enhancer. -
Tissue specificity
Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. -
Sequence similarities
Contains 1 homeobox DNA-binding domain.
Contains 2 LIM zinc-binding domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3670 Human
- Entrez Gene: 16392 Mouse
- Omim: 600366 Human
- SwissProt: P61371 Human
- SwissProt: P61372 Mouse
- Unigene: 505 Human
- Unigene: 42242 Mouse
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Alternative names
- Insulin gene enhancer protein ISL 1 antibody
- Insulin gene enhancer protein ISL-1 antibody
- Insulin related protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Immunocytochemistry/Immunofluorescence analysis of SH-SY-5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-122431-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)All lanes : Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (unpurified)
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 39 kDa -
![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-251524-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-251523-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
![Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-210993-anti-islet-1-antibody-ep4182-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Immunocytochemistry/Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling Islet 1 with unpurified ab109517.
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![Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-268754-anti-islet-1-antibody-ep4182-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Intracellular Flow Cytometry analysis of SH-SY5Y (human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution (10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-251522-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-251521-anti-islet-1-antibody-ep4182-western-blot.jpg)
Western blot - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517) at 1/50000 dilution (purified) + K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 39 kDaBlocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-251246-anti-islet-1-antibody-ep4182-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate. 10 µg of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-210490-anti-islet-1-antibody-ep4182-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.
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![Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)](https://www.abcam.com/ps/products/109/ab109517/Images/ab109517-13-benefits-of-recombinant-antibodies.png)
Anti-Islet 1 antibody [EP4182] - Neural Stem Cell Marker (ab109517)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (40)
ab109517 has been referenced in 40 publications.
- Hosoya M et al. The common marmoset as suitable nonhuman alternative for the analysis of primate cochlear development. FEBS J 288:325-353 (2021). PubMed: 32323465
- Domínguez-Bautista JA et al. Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord. Front Cell Dev Biol 9:587096 (2021). PubMed: 33575260
- Li Y et al. 4-phenylbutyrate exerts stage-specific effects on cardiac differentiation via HDAC inhibition. PLoS One 16:e0250267 (2021). PubMed: 33882103
- Shi Q et al. Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer. Mol Med 27:47 (2021). PubMed: 33962568
- Huang X et al. Human amyotrophic lateral sclerosis excitability phenotype screen: Target discovery and validation. Cell Rep 35:109224 (2021). PubMed: 34107252