Recombinant Anti-ITLN1 antibody [EPR20311-300] - BSA and Azide free (Capture) (ab252674)
![Anti-ITLN1 antibody [EPR20311-300] - BSA and Azide free (Capture) (ab252674)](https://www.abcam.com/ps/products/252/ab252674/Images/ab252674-1-benefits-of-recombinant-antibodies.png "Anti-ITLN1 antibody [EPR20311-300] - BSA and Azide free (Capture) (ab252674)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20311-300] to ITLN1 - BSA and Azide free (Capture)
- Suitable for: Sandwich ELISA
- Reacts with: Human
Overview
-
Product name
Anti-ITLN1 antibody [EPR20311-300] - BSA and Azide free (Capture)
See all ITLN1 primary antibodies -
Description
Rabbit monoclonal [EPR20311-300] to ITLN1 - BSA and Azide free (Capture) -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab252674 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Omentin. The recommended pair for sandwich ELISA is:
Capture: ab252674, Human Omentin Capture Antibody (unconjugated)
Detector: ab252615, Human Omentin Detector Antibody (unconjugated)
The reference range value is 0.3125 - 20 pg/ml.Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20311-300 -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
sELISA pair antibody
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252674 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [EPR20311-224] to ITLN1 - BSA and Azide free (Detector) (ab252615).
|
| Notes |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [EPR20311-224] to ITLN1 - BSA and Azide free (Detector) (ab252615). |
Target
-
Function
Has no effect on basal glucose uptake but enhances insulin-stimulated glucose uptake in adipocytes. Increases AKT phosphorylation in the absence and presence of insulin. May play a role in the defense system against microorganisms. May specifically recognize carbohydrate chains of pathogens and bacterial components containing galactofuranosyl residues, in a calcium-dependent manner. May be involved in iron metabolism. -
Tissue specificity
Highly expressed in omental adipose tissue where it is found in stromal vascular cells but not in fat cells but is barely detectable in subcutaneous adipose tissue (at protein level). Highly expressed in the small intestine. Also found in the heart, testis, colon, salivary gland, skeletal muscle, pancreas and thyroid and, to a lesser degree, in the uterus, spleen, prostate, lymph node and thymus. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Developmental stage
Found in fetal small intestine and thymus. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Secreted. Enriched in lipid rafts. - Information by UniProt
-
Database links
- Entrez Gene: 55600 Human
- Omim: 609873 Human
- SwissProt: Q8WWA0 Human
- Unigene: 50813 Human
-
Alternative names
- Endothelial lectin HL 1 antibody
- Endothelial lectin HL-1 antibody
- FLJ20022 antibody
see all
Images
-
Anti-ITLN1 antibody [EPR20311-300] - BSA and Azide free (Capture) (ab252674)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab252674 has not yet been referenced specifically in any publications.
