Anti-Jagged1 antibody (ab7771)
Key features and details
- Rabbit polyclonal to Jagged1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Jagged1 antibody
See all Jagged1 primary antibodies -
Description
Rabbit polyclonal to Jagged1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide: N-KASRGNDRNRIVLPFS conjugated to KLH, corresponding to amino acids 110-125 of Human Jagged1
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Positive control
- WB: Mouse liver lysate. IHC: Human cervical and human cervical cancer tissues. ICC/IF: Human corneal epithelial cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7771 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| IHC-P | (4) |
1/100.
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| WB | (2) |
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
1/100. |
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WB
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 134 kDa). 150 kDa in mouse liver lysate. |
Target
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Function
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
Tissue specificity
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
Involvement in disease
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
Sequence similarities
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
Developmental stage
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 182 Human
- Entrez Gene: 16449 Mouse
- Entrez Gene: 29146 Rat
- Omim: 601920 Human
- SwissProt: P78504 Human
- SwissProt: Q9QXX0 Mouse
- SwissProt: Q63722 Rat
- Unigene: 224012 Human
see all -
Alternative names
- AGS antibody
- AHD antibody
- AWS antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast Laboratory
Formalin-fixed paraffin embedded IHC of normal human cervial tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin
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Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody (ab7771)This image is courtesy of Aihua Ma, Univdersity of CardiffStaining of Human corneal epithelial cells with anti-Jagged1 (ab7771, 1/500). The Jagged1 (green staining) is localised to the cytoplasm and cell nucleus and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (1/500, blue staining).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Jagged1 antibody (ab7771)This image is courtesy of the Martin Kast LaboratoryFormalin-fixed paraffin embedded IHC of human cervical cancer tissues (40x magnification) stained with ab7771 (1/100 dilution). The slides were counterstained with hematoxylin -
Western blot - Anti-Jagged1 antibody (ab7771)ab7771 Rabbit polyclonal to Jagged1 (1/500) with secondary Goat anti-rabbit IgG antibody ab6721 (1/5000)
Exposure time: 1 minute. Expected molecular weight: 134 kDaLanes 1 to 3 and 5: 20µg of cell lysate per lane
Lane 4: 20 µl of 1x LDS loading bufferLane 1: Human Brain tissue lysate
Lane 2: Human Kidney tissue Lysate
Lane 3: Human liver tissue lysate
Lane 4: 1x LDS loading buffer
Lane 5: Mouse liver lysate
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (85)
ab7771 has been referenced in 85 publications.
- Liu Y et al. Hsa-miR-599 inhibits breast cancer progression via BRD4/Jagged1/Notch1 axis. J Cell Physiol 237:523-531 (2022). PubMed: 34415065
- Dave JM et al. JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. J Clin Invest 132:N/A (2022). PubMed: 34990407
- Ngo TTT et al. Functional differentiation and scalable production of renal proximal tubular epithelial cells from human pluripotent stem cells in a dynamic culture system. Cell Prolif 55:e13190 (2022). PubMed: 35102634
- Zhang N et al. IL-17 is a Potential Therapeutic Target in a Rodent Model of Otitis Media with Effusion. J Inflamm Res 15:635-648 (2022). PubMed: 35140496
- Xie Y & Chen G LncRNA SNHG12 promotes the malignant progression of melanoma by targeting miR-199b. Ann Transl Med 10:226 (2022). PubMed: 35280401