Recombinant Anti-Jagged1 antibody [EPR26134-17] (ab300561)
![Western blot - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)](https://www.abcam.com/ps/products/300/ab300561/Images/AB300561-487434-anti-jagged1-antibody-epr2613417-western-blot-hepg2-human-hepatocellular-carcinoma-epithelial-cell-whole-cell-lysate20-ug.jpg "Western blot - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26134-17] to Jagged1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Jagged1 antibody [EPR26134-17]
See all Jagged1 primary antibodies -
Description
Rabbit monoclonal [EPR26134-17] to Jagged1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt (Intra) or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Whole cell lysates: HepG2 (human hepatocellular carcinoma epithelial cell), HUVEC (human umbilical vein endothelial cell), Hepa1-6 (mouse hepatoma epithelial cell), bEnd.3 (mouse brain endothelioma), AR42J (rat pancreatic tumor epithelial cell). ICC/IF: Hepa1-6 (mouse hepatoma epithelial cells). IP: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate.
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General notes
ab300561 does not react in immunohistochemistry and intracellular flow cytometry.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26134-17 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000.
|
| ICC/IF |
1/500.
|
|
| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. |
|
ICC/IF
1/500. |
|
IP
1/30. |
Target
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Function
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
Tissue specificity
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
Involvement in disease
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
Sequence similarities
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
Developmental stage
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 182 Human
- Entrez Gene: 16449 Mouse
- Entrez Gene: 29146 Rat
- Omim: 601920 Human
- SwissProt: P78504 Human
- SwissProt: Q9QXX0 Mouse
- SwissProt: Q63722 Rat
- Unigene: 224012 Human
see all -
Alternative names
- AGS antibody
- AHD antibody
- AWS antibody
see all
Images
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Western blot - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)All lanes : Anti-Jagged1 antibody [EPR26134-17] (ab300561) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 200, 23 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative controls: Jurkat, MOLT-4 (PMID: 30231940)
200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).
This blot was developed using a high sensitivity ECL substrate.
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![Western blot - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)](https://www.abcam.com/ps/products/300/ab300561/Images/AB300561-487435-anti-jagged1-antibody-epr2613417-western-blot-hepa16-mouse-hepatoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)All lanes : Anti-Jagged1 antibody [EPR26134-17] (ab300561) at 1/1000 dilution
Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate
Lane 2 : bEnd.3 (mouse brain endothelioma), whole cell lysate
Lane 3 : AR42J (rat pancreatic tumor epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 200, 23 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).
Lane 3 of this blot was developed using a high sensitivity ECL substrate.
Exposure time:
Lane 1: 59 seconds
Lane 2: 169 seconds
Lane 3: 3 minutes
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![Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody [EPR26134-17] (ab300561)](https://www.abcam.com/ps/products/300/ab300561/Images/AB300561-2-anti-jagged1-antibody-epr2613417-immunocytochemistry-hepa16.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Jagged1 antibody [EPR26134-17] (ab300561)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cells) labeling Jagged1 with ab300561 at 1/500 dilution (1.04 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing cytoplasmic and membrane staining in Hepa1-6 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: PBS was used instead of primary antobody, followed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml).
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![Immunoprecipitation - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)](https://www.abcam.com/ps/products/300/ab300561/Images/AB300561-3-anti-jagged1-antibody-epr2613417-immunoprecipitation-hepa16.jpg)
Immunoprecipitation - Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)Jagged1 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) 10 μg, whole cell lysate with ab300561 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate 10 ug
Lane 2: ab300561 IP in Hepa1-6 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300561 in Hepa1-6 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).
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![Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)](https://www.abcam.com/ps/products/300/ab300561/Images/AB300561-1-Benefits-of-Recombinant-antibodies.jpg)
Rabbit monoclonal [EPR26134-17] to Jagged1 (AB300561)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300561 has not yet been referenced specifically in any publications.