Recombinant Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
![Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)](https://www.abcam.com/ps/products/232/ab232632/Images/ab232632-322480-ab2326322ab183714216194Picture8.jpg "Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-JAML antibody [EPR15289] - BSA and Azide free
See all JAML primary antibodies -
Description
Rabbit monoclonal [EPR15289] to JAML - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: C6 and RAW 264.7 whole cell lysates.
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General notes
ab232632 is the carrier-free version of ab183714.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15289 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa).
|
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 44 kDa). |
|
IP
Use at an assay dependent concentration. |
Target
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Function
May function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells. -
Tissue specificity
Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Domain
The Ig-like V-type domain 2 mediates interaction with CXADR. -
Cellular localization
Cell junction. Cell membrane. Localized at the plasma membrane in areas of cell-cell contacts and at the cell surface of PMN. - Information by UniProt
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Database links
- Entrez Gene: 120425 Human
- Entrez Gene: 270152 Mouse
- Entrez Gene: 315610 Rat
- Omim: 609770 Human
- SwissProt: Q86YT9 Human
- SwissProt: Q80UL9 Mouse
- Unigene: 16291 Human
- Unigene: 190461 Mouse
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Alternative names
- adhesion molecule AMICA antibody
- Adhesion molecule interacting with CXADR antigen 1 antibody
- adhesion molecule interacts with CXADR antigen 1 antibody
see all
Images
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Immunoprecipitation - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
Western blot analysis of JAML in 293 cell lysate immunoprecipitated using ab183714 at 1/50 dilution (Lane 1). Lane 2 : TBS instead of 293 lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).
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![Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)](https://www.abcam.com/ps/products/232/ab232632/Images/ab232632-308791-anti-jaml-antibody-epr15289-western-blot.jpg)
Western blot - Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)All lanes : Anti-JAML antibody [EPR15289] (ab183714) at 1/5000 dilution
Lane 1 : C6 (rat glioma cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 44 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183714).
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![Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)](https://www.abcam.com/ps/products/232/ab232632/Images/ab232632-3-benefits-of-recombinant-antibodies.png)
Anti-JAML antibody [EPR15289] - BSA and Azide free (ab232632)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232632 has not yet been referenced specifically in any publications.
