Recombinant Anti-JNK1 antibody [EPR17557] KO Tested (ab199380)

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Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17557] to JNK1
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, African green monkey, Xenopus tropicalis

Carrier Free

Product name

Anti-JNK1 antibody [EPR17557]
See all JNK1 primary antibodies
Description

Rabbit monoclonal [EPR17557] to JNK1
Host species

Rabbit
Tested applications

Suitable for: WBmore details
Species reactivity

Reacts with: Mouse, Rat, Chicken, Hamster, Dog, Human, African green monkey, Xenopus tropicalis
Predicted to work with: Monkey
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Full length human JNK1 recombinant protein; HeLa, U937, MCF7, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, MDCK, COS-1, UMNSAH/DF-1 and BHK whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Rat brain, heart and spleen lysates; Xenopus (X. tropicalis) muscle lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17557
Isotype

IgG
Research areas
- Neuroscience
- Development

Alternative Versions
- Anti-JNK1 antibody [EPR17557] - BSA and Azide free (ab251271)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Mouse JNK1 protein (ab43627)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2500. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 44 kDa).

Notes
WB 1/2500. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 44 kDa).

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
Target information above from: UniProt accession P45983 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 423778 Chicken
- Entrez Gene: 5599 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 116554 Rat
- Omim: 601158 Human
- SwissProt: P45983 Human
- SwissProt: Q91Y86 Mouse
- SwissProt: P49185 Rat
- Unigene: 138211 Human
- Unigene: 21495 Mouse
- Unigene: 4090 Rat
see all
Alternative names
- AI849689 antibody
- c Jun N terminal kinase 1 antibody
- C-JUN kinase 1 antibody
- c-Jun N-terminal kinase 1 antibody
- EC 2.7.11.24 antibody
- JNK 1 antibody
- JNK antibody
- JNK-46 antibody
- JNK1A2 antibody
- JNK21B1/2 antibody
- MAP kinase 8 antibody
- MAPK 8 antibody
- mapk8 antibody
- Mitogen activated protein kinase 8 antibody
- Mitogen-activated protein kinase 8 antibody
- MK08_HUMAN antibody
- p54 gamma antibody
- Prkm8 antibody
- Protein kinase JNK1 antibody
- Protein kinase, mitogen-activated, 8 antibody
- SAPK 1 antibody
- SAPK gamma antibody
- SAPK1 antibody
- Stress-activated protein kinase 1 antibody
- Stress-activated protein kinase JNK1 antibody
see all

Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : Wild-type U-2 OS cell lysate
Lane 2 : MAPK8 knockout U-2 OS cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 44 kDa
Observed band size: 42-48 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-JNK1 antibody [EPR17557] staining at 1/2500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab199380 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: MCF7 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab199380 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

ab199380 was shown to recognize JNK1 when JNK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and JNK1 knockout samples were subjected to SDS-PAGE. ab199380 and ab8226 (loading control to beta Actin) were diluted to 1/2500 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

Lane 1 : Full length Human JNK1 recombinant protein
Lane 2 : Full length Human JNK2 recombinant protein
Lane 3 : Full length Human JNK3 recombinant protein

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : U937 (Human histiocytic lymphoma cells) whole cell lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution + Human fetal brain lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/5000 dilution

Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat spleen lysate
Lane 4 : C6 (Rat glial tumor cells) whole cell lysate
Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : MDCK (Canine kidney cell line) whole cell lysates
Lane 2 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates
Lane 3 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JNK1 antibody [EPR17557] (ab199380)

All lanes : Anti-JNK1 antibody [EPR17557] (ab199380) at 1/2500 dilution

Lane 1 : Xenopus (X. tropicalis) muscle lysates
Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Observed band size: 46,54 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-JNK1 antibody [EPR17557] (ab199380)

Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

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Publishing research using ab199380? Please let us know so that we can cite the reference in this datasheet.

ab199380 has been referenced in 48 publications.

Sun T et al. Network Pharmacology-Based Strategy Combined with Molecular Docking and in vitro Validation Study to Explore the Underlying Mechanism of Huo Luo Xiao Ling Dan in Treating Atherosclerosis. Drug Des Devel Ther 16:1621-1645 (2022). PubMed: 35669282
Dong W et al. Wnt4 is crucial for cardiac repair by regulating mesenchymal-endothelial transition via the phospho-JNK/JNK. Theranostics 12:4110-4126 (2022). PubMed: 35673578
Gao P et al. S1PR1 regulates NDV-induced IL-1β expression via NLRP3/caspase-1 inflammasome. Vet Res 53:58 (2022). PubMed: 35854395
Yu W et al. TRPV3 inhibits colorectal cancer cell proliferation and migration by regulating the MAPK signaling pathway. J Gastrointest Oncol 13:2447-2457 (2022). PubMed: 36388700
Wang F et al. Pharmacological mechanisms of Fuzheng Huayu formula for Aristolochic acid I-induced kidney fibrosis through network pharmacology. Front Pharmacol 13:1056865 (2022). PubMed: 36569327

View all Publications for this product

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Post-translationalmodifications

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (48)

Customer reviews and Q&As

Post-translational
modifications