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RecombinantRabMAb

Recombinant Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

Reviews (1) Submit a question References (61)
Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
  • Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1597Y] to JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223)
  • Suitable for: Dot blot, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y]

  • Description

    Rabbit monoclonal [EP1597Y] to JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223)

  • Host species

    Rabbit

  • Specificity

    Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).

  • Tested applications

    Suitable for: Dot blot, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa cell lysate; WB: Lysate from UV-treated HeLa, HEK-293, NIH/3T3, and C6 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot
Use at an assay dependent concentration.
WB (1)
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/5000 - 1/20000.
IP
1/70.

For unpurified use at 1/20.
Notes
Dot blot
Use at an assay dependent concentration.
WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/5000 - 1/20000.
IP
1/70.

For unpurified use at 1/20.

Images

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Lanes 1-6 : Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/5000 dilution (Purified)


    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates
    Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) treated with 200J/m2 UV-C then recovery for 30 minutes whole cell lysates
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
    Lane 6 : C6 (Rat glial tumor glial cell) treated with 40J/m2 UV-C then recovery for 30 minutes whole cell lysates


    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer: 5% NFDM/TBST

  • Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Immunoprecipitation - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Purified ab76572 at 1/70 dilution (2ug) immunoprecipitating JNK1+JNK2+JNK3 in HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate (10µg)
    Lane 2 (+): ab76572 + HeLa treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab76572 in HeLa treated with 20J/m2 UV-C then recovery for 1h whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 46, 54 kDa

  • Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Western blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    All lanes : Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572) at 1/2000 dilution (unpurified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates with 5% NFDM/TBST
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates with 5% NFDM/TBST
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour, whole cell lysates. Then the membrane was incubated with alkaline phosphatase with 5% NFDM/TBST
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 20J/m2 UV-C then recovery for 1 hour whole cell lysates. Then the membrane was incubated with lambda phosphatase with 5% NFDM/TBST

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Dot Blot - Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

    Lane 1: Human JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) phospho peptide
    Lane 2: Human JNK1+JNK2+JNK3 non-phospho pepetide

    Stained with unpurified ab76572 at a 1:1000 dilution. Dection was with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051)  at a 1:100,000 dilution.

    Exposure: 3 minutes

  • Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)
    Anti-JNK1 (phospho Y185) + JNK2 (phospho Y185) + JNK3 (phospho Y223) antibody [EP1597Y] (ab76572)

References (61)

Publishing research using ab76572? Please let us know so that we can cite the reference in this datasheet.

ab76572 has been referenced in 61 publications.

  • Yang D  et al. Sinensetin attenuates oxygen-glucose deprivation/reperfusion-induced neurotoxicity by MAPK pathway in human cerebral microvascular endothelial cells. J Appl Toxicol 42:683-693 (2022). PubMed: 34664717
  • Zhuang Q  et al. Knockdown of circ-RAD23B inhibits non-small cell lung cancer progression via the miR-142-3p/MAP4K3 axis. Thorac Cancer 13:750-760 (2022). PubMed: 35106926
  • Willis A  et al. Enzymatic Degradation of Cortical Perineuronal Nets Reverses GABAergic Interneuron Maturation. Mol Neurobiol 59:2874-2893 (2022). PubMed: 35233718
  • Gao X  et al. Piceatannol suppresses inflammation and promotes apoptosis in rheumatoid arthritis‑fibroblast‑like synoviocytes by inhibiting the NF‑κB and MAPK signaling pathways. Mol Med Rep 25:N/A (2022). PubMed: 35322865
  • Zhang RZ  et al. Qi Sui Zhu Shui Plaster Inhibits AQP1 and MAPK Signaling Reduces Liver Damage Induced by Cirrhotic Ascites. J Healthc Eng 2022:9928546 (2022). PubMed: 35399826
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-JNK1 + JNK2 + JNK1 + JNK2 + JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary rat hepatocyte)
Gel Running Conditions
Reduced Denaturing (9%)
Loading amount
20 µg
Treatment
1mM paracetamol
Specification
Primary rat hepatocyte
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Philip Probert

Verified customer

Submitted Jun 03 2016

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