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    products/primary-antibodies/junctional-adhesion-molecule-1jam-a-antibody-ep1042y-ab52647.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (19)

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Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
  • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
  • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1/JAM-A
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Primary
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Anti-GBA antibody [EPR5142] (ab125065)
Protein
Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein (ab151859)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies
  • Description

    Rabbit monoclonal [EP1042Y] to Junctional Adhesion Molecule 1/JAM-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Junctional Adhesion Molecule 1/JAM-A aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Hela cell lysate and human liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1042Y
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell junction molecules

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab224986)
    • Alexa Fluor® 488 Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab224987)
    • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] - BSA and Azide free (ab232338)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant Human Junctional Adhesion Molecule 1/JAM-A protein (ab151859)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
  • Target information above from: UniProt accession Q9Y624 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 50848 Human
    • Omim: 605721 Human
    • SwissProt: Q9Y624 Human
    • Unigene: 517293 Human
    • Alternative names

      • CD 321 antibody
      • CD321 antibody
      • CD321 antigen antibody
      • ESTM33 antibody
      • F11 receptor antibody
      • F11R antibody
      • JAM 1 antibody
      • JAM A antibody
      • JAM antibody
      • JAM-1 antibody
      • JAM-A antibody
      • JAM1 antibody
      • JAM1_HUMAN antibody
      • JAMA antibody
      • JCAM antibody
      • Jcam1 antibody
      • Junction adhesion molecule 1 antibody
      • Junction adhesion molecule, mouse, homolog of antibody
      • Junctional adhesion molecule 1 antibody
      • Junctional adhesion molecule A antibody
      • KAT antibody
      • Ly106 antibody
      • PAM 1 antibody
      • PAM-1 antibody
      • PAM1 antibody
      • Platelet adhesion molecule 1 antibody
      • Platelet adhesion molecule antibody
      • Platelet F11 receptor antibody
      • PRO301 antibody
      • UNQ264 antibody
      see all

    Images

    • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

      Lane 1: Wild-type HAP1 whole cell lysate (40 µg)
      Lane 2: Junctional Adhesion Molecule 1/JAM-A knockout HAP1 whole cell lysate (40 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab52647 observed at 32 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab52647 was shown to specifically react with Junctional Adhesion Molecule 1/JAM-A in wild-type HAP1 cells as signal was lost in Junctional Adhesion Molecule 1 knockout cells. Wild-type and Junctional Adhesion Molecule 1/JAM-A knockout samples were subjected to SDS-PAGE. ab52647 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Junctional Adhesion Molecule 1/JAM-A with Purified ab52647 at 1:100 dilution (6.15 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647) at 1/1000 dilution (purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 33 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Western blot - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647) at 1/2000 dilution (unpurified) + Hela cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

      Immunohistochemical staining of paraffin-embedded human liver using unpurified ab52647 at a 1/100 dilution.

    • Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)
      Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y] (ab52647)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab52647? Please let us know so that we can cite the reference in this datasheet.

    ab52647 has been referenced in 19 publications.

    • Murakami T  et al. Aberrant expression of junctional adhesion molecule-A contributes to the malignancy of cervical adenocarcinoma by interaction with poliovirus receptor/CD155. Cancer Sci 112:906-917 (2021). PubMed: 33185939
    • Yang C  et al. Genetic Deletion or Pharmacological Inhibition of Cyclooxygenase-2 Reduces Blood-Brain Barrier Damage in Experimental Ischemic Stroke. Front Neurol 11:887 (2020). PubMed: 32973660
    • Jing W  et al. Phosphate Binder, Ferric Citrate, Attenuates Anemia, Renal Dysfunction, Oxidative Stress, Inflammation, and Fibrosis in 5/6 Nephrectomized CKD Rats. J Pharmacol Exp Ther 367:129-137 (2018). PubMed: 30093458
    • Jing W  et al. Uremia induces upregulation of cerebral tissue oxidative/inflammatory cascade, down-regulation of Nrf2 pathway and disruption of blood brain barrier. Am J Transl Res 10:2137-2147 (2018). PubMed: 30093950
    • Hou D  et al. The potential atheroprotective role of plant MIR156a as a repressor of monocyte recruitment on inflamed human endothelial cells. J Nutr Biochem 57:197-205 (2018). PubMed: 29751293
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Julien Chevalier

    Verified customer

    Submitted Nov 18 2015

    Western blot abreview for Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (Intestinal tissue (mucosa and submucosa))
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue (mucosa and submucosa)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Julien Chevalier

    Verified customer

    Submitted Oct 16 2015

    Western blot abreview for Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EP1042Y]

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Intestinal tissue)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis Tris MOPS)
    Loading amount
    14 µg
    Specification
    Intestinal tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Julien Chevalier

    Verified customer

    Submitted Oct 12 2015

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