Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-JunD antibody [EPR17365] (ab181615)

Reviews (1) Submit a question References (6)
Western blot - Anti-JunD antibody [EPR17365] (ab181615)
  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] (ab181615)
  • Anti-JunD antibody [EPR17365] (ab181615)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17365] to JunD
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-JunD antibody [EPR17365]
    See all JunD primary antibodies

  • Description

    Rabbit monoclonal [EPR17365] to JunD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, Jurkat, HeLa, C6, Raw264.7, PC12, NIH 3T3 cell lysates; Human fetal liver, fetal brain, fetal heart and fetal kidney lysates. IHC-P: Human mammary gland tissue and lung squamous cell carcinoma tissue; Mouse and rat cerebral cortex tissues. ICC/IF: HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 39,42 kDa (predicted molecular weight: 35 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/10000.
Notes
WB
1/1000. Detects a band of approximately 39,42 kDa (predicted molecular weight: 35 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/10000.

Target

Images

  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

    Lane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
    Lane 3 : Human fetal liver lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    5% NFDM/TBST: Blocking and diluting buffer.

  • Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    Western blot - Anti-JunD antibody [EPR17365] (ab181615)
    All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
    Lane 4 : NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa
    Observed band size: 39,42 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    5% NFDM/TBST: Blocking and diluting buffer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

    Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nuclear staining on the epithelial cells of Human mammary gland was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

    Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

    Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nuclear staining on neurons of the mouse cerebral cortex was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
    Note: Nuclear staining on neurons of the rat cerebral cortex was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] (ab181615)
    Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] (ab181615)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, labeling JunD with ab181615 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    1. ab181615 at 1/10000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Anti-JunD antibody [EPR17365] (ab181615)
    Anti-JunD antibody [EPR17365] (ab181615)

References (6)

Publishing research using ab181615? Please let us know so that we can cite the reference in this datasheet.

ab181615 has been referenced in 6 publications.

  • Marfella R  et al. Sodium/glucose cotransporter 2 (SGLT2) inhibitors improve cardiac function by reducing JunD expression in human diabetic hearts. Metabolism 127:154936 (2022). PubMed: 34801581
  • Wang K  et al. JunD Regulates Pancreatic ß-Cells Function by Altering Lipid Accumulation. Front Endocrinol (Lausanne) 12:689845 (2021). PubMed: 34335468
  • Kuonen F  et al. c-FOS drives reversible basal to squamous cell carcinoma transition. Cell Rep 37:109774 (2021). PubMed: 34610301
  • Wasylishen AR  et al. Men1 maintains exocrine pancreas homeostasis in response to inflammation and oncogenic stress. Proc Natl Acad Sci U S A 117:6622-6629 (2020). PubMed: 32156729
  • Shirali AS  et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). PubMed: 29615716
  • Liu F  et al. The role of NF-?B-mediated JNK pathway in cognitive impairment in a rat model of sleep apnea. J Thorac Dis 10:6921-6931 (2018). PubMed: 30746238

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

Flow Cytometry abreview for Anti-JunD antibody [EPR17365]

 Average
Abreviews
abreview image
Application
Flow Cytometry
Sample
Mouse Cell (Mouse Breast Cancer Cell Line (4T.2))
Permeabilization
Yes - eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set
Gating Strategy
morphology --> single cells --> gated on live cells (via Zombie Aqua™ Fixable Viability Kit staining) --> and then assessed JunD expression
Specification
Mouse Breast Cancer Cell Line (4T.2)
Preparation
Cell harvesting/tissue preparation method: Thawed frozen vial of 4T1.2 cells (stored in FCS with 10% DMSO) and grew in alpha-MEM until confluent.
Sample buffer: FACS buffer
Fixation
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 28 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com