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    products/primary-antibodies/kappa-light-chain-antibody-epr5367-8-ab124727.pdf

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Immunology Immunoglobulins Light Chain Kappa
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RecombinantRabMAb

Recombinant Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (1)

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ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
  • Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
  • Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
  • Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-8] to Kappa light chain
  • Suitable for: WB, IP, ELISA, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Primary
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Anti-Human IgG Lambda Light Chain antibody [RM127] (ab195573)
Primary
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HRP Anti-Kappa light chain antibody [EPR5367-8] (ab202549)

View more associated products

Overview

  • Product name

    Anti-Kappa light chain antibody [EPR5367-8]
    See all Kappa light chain primary antibodies
  • Description

    Rabbit monoclonal [EPR5367-8] to Kappa light chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.

  • Positive control

    • IP: Human spleen lysate. WB: Human tonsil, plasma and spleen lysate; IHC: Human tonsil lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5367-8
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Kappa

Associated products

  • Alternative Versions

    • HRP Anti-Kappa light chain antibody [EPR5367-8] (ab202549)
    • Alexa Fluor® 647 Anti-Kappa light chain antibody [EPR5367-8] (ab202832)
    • Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
IP
1/10 - 1/100.
ELISA
1/16000.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

antigen retrieval is recommended.

Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
IP
1/10 - 1/100.
ELISA
1/16000.
IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

antigen retrieval is recommended.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

    • Entrez Gene: 28901 Human
    • Entrez Gene: 3514 Human
    • Omim: 147200 Human
    • SwissProt: P01601 Human
    • SwissProt: P01834 Human
    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Images

    • ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      All lanes : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution (Purified)

      Lane 1 : Human tonsil lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

      Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
      Lane 1 (input): Human spleen lysate 10µg
      Lane 2 (+): ab124727 + Human spleen lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 25 kDa

    • Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
      Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab124727? Please let us know so that we can cite the reference in this datasheet.

    ab124727 has been referenced in 1 publication.

    • Deng Z  et al. Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33760139

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Nous achetons actuellement un Anti-Corps anti-IgG Humain chez Sigma (voir certificat ci-joint). Nous voudrions savoir si vous fabriquer aussi cet anti-corps. Pourriez-vous nous envoyer alors un devis, SVP? Quel serait le délai de livraison?
    Par avance, merci,
    Cordialement,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 20 2012

    Answer

    Merci de nous avoir contactés.

    Nous avons un anticorps semblable à l'anticorps A7164. C'est un monoclonal de lapin anti-Human IgG Kappa Light Chain, clone[EPR5367], référence ab124727, https://www.abcam.com/ab124727. Notez que cet anticorps n'est non-conjugué.

    Nous avons également d'autres anti-IgG humain, non-conjugués ou conjugués:


    https://www.abcam.com/Human-IgG-antibody-ab2410.html
    https://www.abcam.com/Human-IgG-antibody-ab2410.html
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    https://www.abcam.com/Human-IgG-antibody-EPR4421-ab109489.htmlhttps://www.abcam.com/Human-IgG-antibody-EPR4421-ab109489.html
    https://www.abcam.com/Human-IgG-antibody-EPR4421-ab109489.html
    Rabbit monoclonal

    https://www.abcam.com/Human-IgG-antibody-2A11-ab7497.html
    https://www.abcam.com/Human-IgG-antibody-2A11-ab7497.html
    Mouse monoclonal

    https://www.abcam.com/Human-IgG-antibody-FITC-ab4220.html
    https://www.abcam.com/Human-IgG-antibody-FITC-ab4220.html
    Rabbit polyclonal
    FITC conjugated

    https://www.abcam.com/Human-IgG-antibody-prediluted-ab938.html
    https://www.abcam.com/Human-IgG-antibody-prediluted-ab938.html
    Rabbit polyclonal

    https://www.abcam.com/Human-IgG-antibody-FITC-ab81051.html
    https://www.abcam.com/Human-IgG-antibody-FITC-ab81051.html
    Goat polyclonal
    FITC conjugated

    https://www.abcam.com/Human-IgG-antibody-08C05-ab72528.html
    https://www.abcam.com/Human-IgG-antibody-08C05-ab72528.html
    Mouse monoclonal

    https://www.abcam.com/Human-IgG-antibody-CH1-ab7500.html
    https://www.abcam.com/Human-IgG-antibody-CH1-ab7500.html
    Mouse monoclonal

    https://www.abcam.com/Human-IgG-antibody-3D3-ab7498.html
    https://www.abcam.com/Human-IgG-antibody-3D3-ab7498.html
    Mouse monoclonal

    https://www.abcam.com/human-IgG-Fab-fragment-antibody-4A11-ab771.html
    https://www.abcam.com/human-IgG-Fab-fragment-antibody-4A11-ab771.html
    Mouse monoclonal

    https://www.abcam.com/Human-IgG-Lambda-Light-Chain-antibody-EPR5367-ab124719.htmlhttps://www.abcam.com/Human-IgG-Lambda-Light-Chain-antibody-EPR5367-ab124719.html
    https://www.abcam.com/Human-IgG-Lambda-Light-Chain-antibody-EPR5367-ab124719.html
    Rabbit monoclonal

    https://www.abcam.com/Human-IgG-Fc-antibody-ABM122-ab125258.html
    https://www.abcam.com/Human-IgG-Fc-antibody-ABM122-ab125258.html
    Mouse monoclonal

    https://www.abcam.com/Human-Kappa-Chain-antibody-EPR5539-ab134929.htmlhttps://www.abcam.com/Human-Kappa-Chain-antibody-EPR5539-ab134929.html
    https://www.abcam.com/Human-Kappa-Chain-antibody-EPR5539-ab134929.html
    Rabbit monoclonal

    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Biotin-ab79114.html
    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Biotin-ab79114.html
    Mouse monoclonal
    Biotin conjugated

    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-HRP-ab79115.html
    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-HRP-ab79115.html
    Mouse monoclonal
    HRP conjugated

    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Alkaline-Phosphatase-ab79117.html
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    Mouse monoclonal
    Alkaline Phosphatase conjugated

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    Mouse monoclonal
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    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Allophycocyanin-ab79112.html
    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Allophycocyanin-ab79112.html
    Mouse monoclonal
    Allophycocyanin conjugated

    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Phycoerythrin-ab79113.html
    https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Phycoerythrin-ab79113.html
    Mouse monoclonal
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    Read More

    Abcam Scientific Support

    Answered on Sep 20 2012

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