Recombinant Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
![ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.com/ps/products/124/ab124727/Images/ab124727-8-anti-kappa-light-chain-antibody-epr5367-8-elisa.jpg "ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5367-8] to Kappa light chain
- Suitable for: WB, IP, ELISA, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Kappa light chain antibody [EPR5367-8]
See all Kappa light chain primary antibodies -
Description
Rabbit monoclonal [EPR5367-8] to Kappa light chain -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.
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Positive control
- IP: Human spleen lysate. WB: Human tonsil, plasma and spleen lysate; IHC: Human tonsil lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5367-8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
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| IP |
1/10 - 1/100.
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| ELISA |
1/16000.
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended. |
| Notes |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 25 kDa. |
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IP
1/10 - 1/100. |
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ELISA
1/16000. |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. antigen retrieval is recommended. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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ELISA - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.com/ps/products/124/ab124727/Images/ab124727-7-anti-kappa-light-chain-antibody-epr5367-8-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.com/ps/products/124/ab124727/Images/ab124727-458205-anti-kappa-light-chain-antibody-epr5367-8-westernblot-human.jpg)
Western blot - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)All lanes : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution (Purified)
Lane 1 : Human tonsil lysate
Lane 2 : Human plasma lysate
Lane 3 : Human spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 25 kDa -
![Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.com/ps/products/124/ab124727/Images/ab124727-5-anti-kappa-light-chain-antibody-epr5367-8-immunoprecipitation-spleen-human.jpg)
Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] (ab124727)Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
Lane 1 (input): Human spleen lysate 10µg
Lane 2 (+): ab124727 + Human spleen lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 25 kDa -
![Anti-Kappa light chain antibody [EPR5367-8] (ab124727)](https://www.abcam.com/ps/products/124/ab124727/Images/ab124727-3-benefits-of-recombinant-antibodies.png)
Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124727 has been referenced in 2 publications.
- Flisikowska T et al. A humanized minipig model for the toxicological testing of therapeutic recombinant antibodies. Nat Biomed Eng 6:1248-1256 (2022). PubMed: 36138193
- Deng Z et al. Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33760139