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RecombinantRabMAb

Recombinant Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)

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ELISA - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
  • Western blot - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
  • Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
  • Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-8] to Kappa light chain - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB, ELISA
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 HRP Unconjugated

Overview

  • Product name

    Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free
    See all Kappa light chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-8] to Kappa light chain - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WB, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA

  • Positive control

    • IP: Human spleen lysate. WB: Human tonsil, plasma and spleen lysate; IHC: Human tonsil lysate.

  • General notes

    ab232152 is the carrier-free version of ab124727.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-8

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab232152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
ELISA
Use at an assay dependent concentration.
Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.
WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

  • Cellular localization

    Cytoplasmic and Secreted

  • Database links

    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Images

    • ELISA - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      ELISA - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      This data was developed using ab124727, the same antibody clone in a different buffer formulation.
      ELISA analysis of Kappa Light Chains, Lambda Light Chains, Human IgA, Human IgM, Human IgG, Rat IgG,and Mouse IgG at 1000ng/mL using purified ab124727. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1/2500 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      This data was developed using ab124727, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with purified ab124727 at 1/5000 dilution (0.06 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      Western blot - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      Lane 1 : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution (Purified)
      Lanes 2-3 : Anti-Kappa light chain antibody [EPR5367-8] (ab124727) at 1/5000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 25 kDa



      This data was developed using ab124727, the same antibody clone in a different buffer formulation.

    • Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      Immunoprecipitation - Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)

      This data was developed using ab124727, the same antibody clone in a different buffer formulation.
      Purified ab124727 at 1/50 dilution (2µg) immunoprecipitating Kappa light chain in Human spleen lysate.
      Lane 1 (input): Human spleen lysate 10µg
      Lane 2 (+): ab124727 + Human spleen lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab124727 in Human spleen lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 25 kDa

    • Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)
      Anti-Kappa light chain antibody [EPR5367-8] - BSA and Azide free (ab232152)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab232152? Please let us know so that we can cite the reference in this datasheet.

    ab232152 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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