Recombinant Anti-KAT5 / Tip60 antibody [EPR23728-112] (ab300521)
![Immunocytochemistry/ Immunofluorescence - Anti-KAT5 / Tip60 antibody [EPR23728-112] (ab300521)](https://www.abcam.com/ps/products/300/ab300521/Images/AB300521-486415-anti-KAT5--Tip60-EPR23728112-ICC-HCT116.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KAT5 / Tip60 antibody [EPR23728-112] (ab300521)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23728-112] to KAT5 / Tip60
- Suitable for: ICC/IF, WB, Flow Cyt (Intra), IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KAT5 / Tip60 antibody [EPR23728-112]
See all KAT5 / Tip60 primary antibodies -
Description
Rabbit monoclonal [EPR23728-112] to KAT5 / Tip60 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, Flow Cyt (Intra), IPmore details
Unsuitable for: ChIP or IHC-P -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and 293T whole cell lysate; HCT 116 nuclear fraction. ICC/IF: HCT 116 cells. Flow Cyt (Intra): HCT 116 cells. IP: HCT 116 nuclear fraction.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23728-112 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
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| WB |
1/1000. Predicted molecular weight: 58 kDa.
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| Flow Cyt (Intra) |
1/50.
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| IP |
1/30.
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| Notes |
|---|
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ICC/IF
1/50. |
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WB
1/1000. Predicted molecular weight: 58 kDa. |
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Flow Cyt (Intra)
1/50. |
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IP
1/30. |
Target
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Function
Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. -
Sequence similarities
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger. -
Post-translational
modificationsSumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation. -
Cellular localization
Nucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 10524 Human
- Omim: 601409 Human
- SwissProt: Q92993 Human
- Unigene: 397010 Human
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Alternative names
- 60 kDa Tat interactive protein antibody
- 60 kDa Tat-interactive protein antibody
- cPLA(2) interacting protein antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KAT5 / Tip60 antibody [EPR23728-112] (ab300521)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cells) labelling KAT5 / Tip60 with ab300521 at 1/50 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 dilution (green). Confocal image showing nuclear staining in HCT 116 cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (red) at 1/200 dilution.
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![Western blot - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)](https://www.abcam.com/ps/products/300/ab300521/Images/AB300521-486419-anti-KAT5--Tip60-EPR23728112-WB-human.jpg)
Western blot - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)All lanes : Anti-KAT5 / Tip60 antibody [EPR23728-112] (ab300521) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 60, 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking / Diluting buffer: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate.
Lysates were freshly made and used immediately to minimize protein degradation.
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![Western blot - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)](https://www.abcam.com/ps/products/300/ab300521/Images/AB300521-486418-anti-KAT5--Tip60-EPR23728112-WB-hct116.jpg)
Western blot - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)All lanes : Anti-KAT5 / Tip60 antibody [EPR23728-112] (ab300521) at 1/1000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) cytoplasmic fraction
Lane 2 : HCT 116 nuclear fraction
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 60, 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking / Diluting buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 33970102).
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![Immunoprecipitation - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)](https://www.abcam.com/ps/products/300/ab300521/Images/AB300521-486416-anti-KAT5--Tip60-EPR23728112-IP-hct116.jpg)
Immunoprecipitation - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)KAT5 / Tip60 was immunoprecipitated from 10 μg HCT 116 (human colorectal carcinoma epithelial cell) nuclear fraction with ab300521 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab300521 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HCT 116 nuclear fraction 10 μg (Input).
Lane 2: HCT 116 nuclear fraction.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300521 in HCT 116 nuclear fraction.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Observed MW(KDa): 60, 50
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![Flow Cytometry (Intracellular) - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)](https://www.abcam.com/ps/products/300/ab300521/Images/AB300521-486414-anti-KAT5--Tip60-EPR23728112-flow-cytometry-HCT116.jpg)
Flow Cytometry (Intracellular) - Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)Flow Cytometry (Intracellular) analysis of HCT 116 (human colorectal carcinoma epithelial cells) labeling KAT5 / Tip60 with ab300521 at a 1/50 dilution (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, (ab150081)) was used as the secondary antibody at a 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlabelled control - Cells without incubation with primary antibody and secondary antibody (blue)
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![Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)](https://www.abcam.com/ps/products/300/ab300521/Images/AB300521-486426-Benefits-of-Recombinant-antibodies.jpg)
Anti-KAT5 / Tip60 antibody [EPR23728-112] (AB300521)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300521 has not yet been referenced specifically in any publications.