Anti-KAT9 / Elp3 antibody (ab113228)
Key features and details
- Rabbit polyclonal to KAT9 / Elp3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT9 / Elp3 antibody
See all KAT9 / Elp3 primary antibodies -
Description
Rabbit polyclonal to KAT9 / Elp3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human Tonsil and Uterus tissues; NT2D1 whole cell lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113228 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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| IHC-P |
Use a concentration of 10 µg/ml.
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| ICC/IF |
1/100 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
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IHC-P
Use a concentration of 10 µg/ml. |
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ICC/IF
1/100 - 1/200. |
Target
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Function
Catalytic histone acetyltransferase subunit of the RNA polymerase II elongator complex, which is a component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4. May also have a methyltransferase activity. -
Sequence similarities
Belongs to the ELP3 family.
Contains 1 N-acetyltransferase domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55140 Human
- Entrez Gene: 74195 Mouse
- Entrez Gene: 541509 Zebrafish
- Omim: 612722 Human
- SwissProt: Q9H9T3 Human
- SwissProt: Q9CZX0 Mouse
- SwissProt: Q5RIC0 Zebrafish
- Unigene: 491336 Human
see all -
Alternative names
- DKFZp761L098 antibody
- Elongation protein 3 homolog (S. cerevisiae) antibody
- Elongation protein 3 homolog antibody
see all
Images
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Western blot - Anti-KAT9 / Elp3 antibody (ab113228)Anti-KAT9 / Elp3 antibody (ab113228) at 1/2000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (ab113228)ab113228, at 10µg/ml, staining KAT9 / Elp3 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT9 / Elp3 antibody (ab113228)ab113228, at 10µg/ml, staining KAT9 / Elp3 in Formalin-fixed, Paraffin-embedded Human Uterus tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-KAT9 / Elp3 antibody (ab113228)ab113228 at 1/100 dilution staining KAT9 / Elp3 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. The lower image is merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab113228 has been referenced in 1 publication.
- Xu S et al. Genome-wide CRISPR screen identifies ELP5 as a determinant of gemcitabine sensitivity in gallbladder cancer. Nat Commun 10:5492 (2019). PubMed: 31792210