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RecombinantRabMAb

Recombinant Anti-KCNIP4 antibody [EPR18314] (ab203831)

Reviews (1) Submit a question References (1)
Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
  • Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)
  • Anti-KCNIP4 antibody [EPR18314] (ab203831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18314] to KCNIP4
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-KCNIP4 antibody [EPR18314]
    See all KCNIP4 primary antibodies

  • Description

    Rabbit monoclonal [EPR18314] to KCNIP4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain lysate; Mouse and rat brain lysates; Rat and mouse cerebellum lysates; Human cerebellum and midbrain lysates. IP: Mouse brain whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab203831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
IP
1/50.
Notes
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
IP
1/50.

Target

  • Function

    Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents.

  • Tissue specificity

    Predominantly expressed in brain.

  • Sequence similarities

    Belongs to the recoverin family.
    Contains 4 EF-hand domains.

  • Domain

    The KIS (K-channel inactivation suppressor) domain is required for converting A-type Kv4 current to a slowly inactivating delayed rectifier potassium current.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • A type potassium channel modulatory protein 4 antibody
    • A-type potassium channel modulatory protein 4 antibody
    • CALP antibody
    • Calsenilin like protein antibody
    • Calsenilin-like protein antibody
    • HGNC:30083 antibody
    • KCHIP 4 antibody
    • KChIP4 antibody
    • KCIP4_HUMAN antibody
    • KCNIP 4 antibody
    • Kcnip4 antibody
    • Kv channel interacting protein 4 antibody
    • Kv channel-interacting protein 4 antibody
    • MGC44947 antibody
    • Potassium channel interacting protein 4 antibody
    • Potassium channel-interacting protein 4 antibody
    see all

Images

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate
    Lane 4 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat heart tissue lysate
    Lane 7 : Rat kidney tissue lysate
    Lane 8 : Rat spleen tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution

    Lane 1 : Rat cerebellum tissue lysate
    Lane 2 : Mouse cerebellum tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Western blot - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human midbrain lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 28 kDa why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Immunoprecipitation - Anti-KCNIP4 antibody [EPR18314] (ab203831)

    KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

    Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab203831 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203831 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-KCNIP4 antibody [EPR18314] (ab203831)
    Anti-KCNIP4 antibody [EPR18314] (ab203831)

References (1)

Publishing research using ab203831? Please let us know so that we can cite the reference in this datasheet.

ab203831 has been referenced in 1 publication.

  • Jenkins CA  et al. Characterisation of canine KCNIP4: A novel gene for cerebellar ataxia identified by whole-genome sequencing two affected Norwegian Buhund dogs. PLoS Genet 16:e1008527 (2020). PubMed: 31999692

Customer reviews and Q&As

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Western blot abreview for Anti-KCNIP4 antibody [EPR18314]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Dog Tissue lysate - whole (Cerebellum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Cerebellum
Blocking step
WesternBreeze blocking solution as blocking agent for 30 minute(s) · Concentration: 30% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Mar 12 2019

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