Recombinant Anti-KCNIP4 antibody [EPR18314] (ab203831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18314] to KCNIP4
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KCNIP4 antibody [EPR18314]
See all KCNIP4 primary antibodies -
Description
Rabbit monoclonal [EPR18314] to KCNIP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain lysate; Mouse and rat brain lysates; Rat and mouse cerebellum lysates; Human cerebellum and midbrain lysates. IP: Mouse brain whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18314 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa).
|
IP |
1/50.
|
Notes |
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WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 29 kDa). |
IP
1/50. |
Target
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Function
Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Probably modulates channels density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. -
Tissue specificity
Predominantly expressed in brain. -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
Domain
The KIS (K-channel inactivation suppressor) domain is required for converting A-type Kv4 current to a slowly inactivating delayed rectifier potassium current. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 80333 Human
- Entrez Gene: 80334 Mouse
- Entrez Gene: 259243 Rat
- Omim: 608182 Human
- SwissProt: Q6PIL6 Human
- SwissProt: Q6PHZ8 Mouse
- SwissProt: Q99MG9 Rat
- Unigene: 655705 Human
see all -
Alternative names
- A type potassium channel modulatory protein 4 antibody
- A-type potassium channel modulatory protein 4 antibody
- CALP antibody
see all
Images
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All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Human fetal spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse heart tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat heart tissue lysate
Lane 7 : Rat kidney tissue lysate
Lane 8 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/1000 dilution
Lane 1 : Rat cerebellum tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human cerebellum lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-KCNIP4 antibody [EPR18314] (ab203831) at 1/10000 dilution + Human midbrain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 29 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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KCNIP4 was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab203831 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab203831 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab203831 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203831 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab203831 has been referenced in 1 publication.
- Jenkins CA et al. Characterisation of canine KCNIP4: A novel gene for cerebellar ataxia identified by whole-genome sequencing two affected Norwegian Buhund dogs. PLoS Genet 16:e1008527 (2020). PubMed: 31999692