Recombinant Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum is observed (PMID:26587966). The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling KCNT1/SLACK with ab305039 at 1/5000 (0.095 µg/ml) followed by a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection). Positive staining on rat spinal cord is observed. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transformed with a KCNT1/SLACK expression vector containing a his tag tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a 6xHis tag construct, no staining is observed on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection). Negative staining on human liver is observed (PMID: 12628167). The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond^™ Polymer Refine Detection) kit.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins. 

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 dilution (2 µg/mL).

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: confocal image showing no staining on mouse liver (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 dilution (2 µg/mL).

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 dilution (2 µg/mL).

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: confocal image showing no staining on rat liver (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60 mins at room temperature. The section was then mounted using Fluoromount^®. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 dilution (2 µg/mL).