Recombinant Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24145-225] to KCNT1/SLACK
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KCNT1/SLACK antibody [EPR24145-225]
See all KCNT1/SLACK primary antibodies -
Description
Rabbit monoclonal [EPR24145-225] to KCNT1/SLACK -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Tissue lysates: Human, mouse and rat cerebrum, rat spinal cord, HEK-293T (human embryonic kidney epithelial cell) transformed with a KCNT1 expression vector containing a his tag. IHC-Fr: Frozen tissues: mouse cerebrum (fresh), rat cerebrum (fresh).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24145-225 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab305039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
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Notes |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. |
Target
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Function
Outwardly rectifying potassium channel subunit that may co-assemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). -
Tissue specificity
Highest expression in liver, brain and spinal cord. Lowest expression in skeletal muscle. -
Sequence similarities
Belongs to the potassium channel family. Calcium-activated (TC 1.A.1.3) subfamily. KCa4.1/KCNT1 sub-subfamily.
Contains 1 RCK N-terminal domain. -
Post-translational
modificationsPhosphorylated by protein kinase C. Phosphorylation of the C-terminal domain increases channel activity. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 57582 Human
- Entrez Gene: 227632 Mouse
- Entrez Gene: 60444 Rat
- Omim: 608167 Human
- SwissProt: Q5JUK3 Human
- SwissProt: Q6ZPR4 Mouse
- SwissProt: Q9Z258 Rat
- Unigene: 104950 Human
see all -
Alternative names
- bA100C15.2 antibody
- EIEE14 antibody
- ENFL5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum is observed (PMID:26587966). The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue labeling KCNT1/SLACK with ab305039 at 1/5000 (0.095 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on rat spinal cord is observed. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transformed with a KCNT1/SLACK expression vector containing a his tag tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Positive staining on (A) HEK-293T transfected with a 6xHis tag construct, no staining is observed on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCNT1/SLACK antibody [EPR24145-225] (ab305039)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling KCNT1/SLACK with ab305039 at 1/5000 dilution (0.095 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Negative staining on human liver is observed (PMID: 12628167). The section was incubated with ab305039 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on mouse cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: confocal image showing no staining on mouse liver (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on rat cerebrum. The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/mL).
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling KCNT1/SLACK with AB305039 at 1/100 dilution (4.74 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: confocal image showing no staining on rat liver (PMID: 24309898). The nuclear counterstain was DAPI (Blue). The section was incubated with ab305039 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/mL).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab305039 has not yet been referenced specifically in any publications.