Anti-KDM2A antibody (ab174693)
Key features and details
- Goat polyclonal to KDM2A
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-KDM2A antibody
See all KDM2A primary antibodies -
Description
Goat polyclonal to KDM2A -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Hamster, Dog, Pig, Monkey
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Immunogen
Synthetic peptide corresponding to Human KDM2A aa 445-459 (internal sequence) (Cysteine residue).
Sequence:PRKDRQVHLTHFELE
Database link: NP_036440.1 -
Positive control
- Human small intestine tissue; Human uterus lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa).
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| IHC-P |
Use a concentration of 5 µg/ml.
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| Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 133 kDa). |
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IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. May also recognize and bind to some phosphorylated proteins and promote their ubiquitination and degradation. Required to maintain the heterochromatic state. Associates with centromeres and represses transcription of small non-coding RNAs that are encoded by the clusters of satellite repeats at the centromere. Required to sustain centromeric integrity and genomic stability, particularly during mitosis. -
Tissue specificity
Widely expressed, with highest levels in brain, testis and ovary, followed by lung. -
Sequence similarities
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 6 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
Domain
The JmjC domain mediates demethylation activity and is required for satellite silencing.
The CXXC zinc finger preferentially recognizes nonmethylated CpG DNA, and binding is blocked when the CpG DNA is methylated. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleoplasm. Punctate expression throughout the nucleoplasm and enriched in the perinucleolar region. Specifically nucleates at CpG islands where it's presence results in chromatin depleted in H3K36me2. - Information by UniProt
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Database links
- Entrez Gene: 483702 Dog
- Entrez Gene: 100052955 Horse
- Entrez Gene: 22992 Human
- Entrez Gene: 225876 Mouse
- Entrez Gene: 100294703 Pig
- Entrez Gene: 361700 Rat
- Omim: 605657 Human
- SwissProt: Q9Y2K7 Human
see all -
Alternative names
- [Histone-H3]-lysine-36 demethylase 1A antibody
- CXXC-type zinc finger protein 8 antibody
- CXXC8 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM2A antibody (ab174693)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling KDM2A with ab174693 at 5 µg/ml.
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Western blot - Anti-KDM2A antibody (ab174693)Anti-KDM2A antibody (ab174693) at 0.5 µg/ml + Human uterus lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 133 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab174693 has been referenced in 2 publications.
- Wang Y et al. p300 Acetylates JHDM1A to inhibit osteosarcoma carcinogenesis. Artif Cells Nanomed Biotechnol 47:2891-2899 (2019). PubMed: 31307234
- Wang F et al. LINC00460 modulates KDM2A to promote cell proliferation and migration by targeting miR-342-3p in gastric cancer. Onco Targets Ther 11:6383-6394 (2018). PubMed: 30323616
