Anti-KDM2B antibody [6F6G11] (ab234082)
![Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307728-anti-kdm2b-antibody-6f6g11-western-blot.jpg "Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)")
Key features and details
- Mouse monoclonal [6F6G11] to KDM2B
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-KDM2B antibody [6F6G11]
See all KDM2B primary antibodies -
Description
Mouse monoclonal [6F6G11] to KDM2B -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human KDM2B aa 457-555. Expressed in E.coli.
Database link: Q8NHM5 -
Positive control
- WB: Human KDM2B (aa 457-555) recombinant protein; KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate; A549 cell lysate. Flow cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6F6G11 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234082 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100 - 1/500. Predicted molecular weight: 153 kDa.
|
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| Flow Cyt |
1/200 - 1/400.
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| Notes |
|---|
|
WB
1/100 - 1/500. Predicted molecular weight: 153 kDa. |
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Flow Cyt
1/200 - 1/400. |
Target
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Function
Histone demethylase that demethylates 'Lys-4' and 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates trimethylated H3 'Lys-4' and dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylated H3 'Lys-36'. Preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which inhibits cell growth and proliferation. May also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. -
Sequence similarities
Belongs to the JHDM1 histone demethylase family.
Contains 1 CXXC-type zinc finger.
Contains 1 F-box domain.
Contains 1 JmjC domain.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 PHD-type zinc finger. -
Domain
The JmjC domain mediates demethylation activity (By similarity). It is also required for repression of ribosomal RNA genes. -
Cellular localization
Nucleus > nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 84678 Human
- Omim: 609078 Human
- SwissProt: Q8NHM5 Human
- Unigene: 524800 Human
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Alternative names
- [Histone-H3]-lysine-36 demethylase 1B antibody
- CXXC-type zinc finger protein 2 antibody
- CXXC2 antibody
see all
Images
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Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)All lanes : Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
Lane 2 : KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate.
Predicted band size: 153 kDa -
![Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307729-anti-kdm2b-antibody-6f6g11-western-blot.jpg)
Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + Human KDM2B (aa 457-555) recombinant protein.
Predicted band size: 153 kDaExpected recombinant MW: 36.8 kDa
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![Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307730-anti-kdm2b-antibody-6f6g11-flow-cytometry.jpg)
Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KDM2B using ab234082 at 1/200 dilution (green) compared to a negative control (red).
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![Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)](https://www.abcam.com/ps/products/234/ab234082/Images/ab234082-307731-anti-kdm2b-antibody-6f6g11-western-blot.jpg)
Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate.
Predicted band size: 153 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab234082 has been referenced in 1 publication.
- Su D et al. Bimodal regulation of the PRC2 complex by USP7 underlies tumorigenesis. Nucleic Acids Res 49:4421-4440 (2021). PubMed: 33849069
