Anti-KDM3A / JHDM2A antibody (ab80598)
Key features and details
- Rabbit polyclonal to KDM3A / JHDM2A
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM3A / JHDM2A antibody
See all KDM3A / JHDM2A primary antibodies -
Description
Rabbit polyclonal to KDM3A / JHDM2A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified using an epitope specific to KDM3A / JHDM2A immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 147 kDa.
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| IP |
Use at 2-5 µg/mg of lysate.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 147 kDa. |
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IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. -
Sequence similarities
Belongs to the JHDM2 histone demethylase family.
Contains 1 JmjC domain. -
Domain
The JmjC domain and the C6-type zinc-finger are required for the demethylation activity.
Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors. -
Cellular localization
Cytoplasm. Nucleus. Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa. - Information by UniProt
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Database links
- Entrez Gene: 55818 Human
- Omim: 611512 Human
- SwissProt: Q9Y4C1 Human
- Unigene: 557425 Human
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Alternative names
- DKFZp686A24246 antibody
- DKFZp686P07111 antibody
- JHDM2A antibody
see all
Images
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Western blot - Anti-KDM3A / JHDM2A antibody (ab80598)All lanes : Anti-KDM3A / JHDM2A antibody (ab80598) at 0.1 µg/ml
Lane 1 : whole HeLa cell lysate at 50 µg
Lane 2 : whole HeLa cell lysate at 15 µg
Lane 3 : whole HeLa cell lysate at 5 µg
Predicted band size: 147 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-KDM3A / JHDM2A antibody (ab80598)Detection of Human KDM3A / JHDM2A by Immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Lane 1: ab80598 used for IP at 3 µg/mg lysate; Lane 2: a control IgG was used for IP. For subsequent Western blot detection, ab80598 was used at 1 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab80598 has been referenced in 3 publications.
- Kong NR et al. Zinc Finger Protein SALL4 Functions through an AT-Rich Motif to Regulate Gene Expression. Cell Rep 34:108574 (2021). PubMed: 33406418
- Ho JC et al. Inhibition of the H3K9 methyltransferase G9A attenuates oncogenicity and activates the hypoxia signaling pathway. PLoS One 12:e0188051 (2017). PubMed: 29145444
- Hickok JR et al. Nitric oxide modifies global histone methylation by inhibiting Jumonji C domain-containing demethylases. J Biol Chem 288:16004-15 (2013). WB ; Human . PubMed: 23546878