Recombinant Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
![Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)](https://www.abcam.com/ps/products/289/ab289667/Images/ab289667-481962-hela.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25241-24] to KDM4D / JMJD2D
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KDM4D / JMJD2D antibody [EPR25241-24]
See all KDM4D / JMJD2D primary antibodies -
Description
Rabbit monoclonal [EPR25241-24] to KDM4D / JMJD2D -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IPmore details
Unsuitable for: ChIP or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human testis tissue lysate; HeLa whole cell lysate. ICC/IF: HeLa cells. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR25241-24 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| ICC/IF |
1/500.
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| WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).
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| IP |
1/30.
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| Notes |
|---|
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Flow Cyt (Intra)
1/500. |
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ICC/IF
1/500. |
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WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa). |
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IP
1/30. |
Target
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Function
Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27', H3 'Lys-36' nor H4 'Lys-20'. Demethylates both di- and trimethylated H3 'Lys-9' residue, while it has no activity on monomethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. -
Sequence similarities
Belongs to the JHDM3 histone demethylase family.
Contains 1 JmjC domain.
Contains 1 JmjN domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55693 Human
- Omim: 609766 Human
- SwissProt: Q6B0I6 Human
- Unigene: 503598 Human
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Alternative names
- JHD3D antibody
- JHDM3D antibody
- JmjC domain-containing histone demethylation protein 3D antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa cells labeling KDM4D / JMJD2D with ab289667 at 1/500 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line. The nuclear counterstain is DAPI (Blue). Tubulin is labeled using ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (Red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)](https://www.abcam.com/ps/products/289/ab289667/Images/ab289667-481960-25241Hela.jpg)
Flow Cytometry (Intracellular) - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human cervix adenocarcinoma epithelial cell line) cells labeling KDM4D / JMJD2D with ab289667 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081)at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)](https://www.abcam.com/ps/products/289/ab289667/Images/ab289667-481958-25241ip1.jpg)
Immunoprecipitation - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)KDM4D / JMJD2D was immunoprecipitated from HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab289667 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab289667 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10 μg (Input).
Lane 2: ab289667 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289667 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)](https://www.abcam.com/ps/products/289/ab289667/Images/ab289667-481956-25241WB2.jpg)
Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667) at 1/1000 dilution + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesLysates were made freshly and used in the WB test immediately to minimize protein degradation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)](https://www.abcam.com/ps/products/289/ab289667/Images/ab289667-481949-25241WB1.jpg)
Western blot - Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)All lanes : Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667) at 1/1000 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : Human ovary tissue lysate
Lane 3 : Human spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG (Merck DC03L) at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes5% NFDM/TBST was used as a dilution and blocking buffer.
Low expression: ovary, spleen (PMID: 21293030).
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![Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)](https://www.abcam.com/ps/products/289/ab289667/Images/ab289667-482313-benefitsofrecombinantantibodies.jpg)
Anti-KDM4D / JMJD2D antibody [EPR25241-24] (ab289667)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab289667 has not yet been referenced specifically in any publications.