Recombinant Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-213089-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg "Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704]
See all KDM5B / PLU1 / Jarid1B primary antibodies -
Description
Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: BxPC-3, MCF7, and HeLa whole cell lysate (ab150035). Mouse and rat testes tissue lysates; ICC/IF: HeLa cells; Flow Cyt (intra): HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12704 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| WB |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).
|
|
| ICC/IF | (1) |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). |
|
ICC/IF
1/50 - 1/100. |
Target
-
Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. -
Tissue specificity
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
Domain
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 10765 Human
- Entrez Gene: 75605 Mouse
- Entrez Gene: 304809 Rat
- Omim: 605393 Human
- SwissProt: Q9UGL1 Human
- SwissProt: Q80Y84 Mouse
- Unigene: 443650 Human
- Unigene: 28995 Mouse
see all -
Alternative names
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
see all
Images
-
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + MCF7 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
![Flow Cytometry (Intracellular) - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-394080-anti-kdm5b-plu1-jarid1b-antibody-epr12704-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Intracellular Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 was used as the secondary antibody (red).Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
-
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-295062-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.
ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
![Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-213090-anti-kdm5b-plu1-jarid1b-antibody-epr12704-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling KDM5B / PLU1 / Jarid1B with ab181089 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter-stained with DAPI (blue).
-
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-213087-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-268956-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/1000 dilution + T47-D (human ductal carcinoma epithelia) whole cell lysate at 20 µg
Secondary
HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-268957-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg)
Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)All lanes : Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089) at 1/5000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG H&L at 1/1000 dilution
Predicted band size: 175 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
-
![Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)](https://www.abcam.com/ps/products/181/ab181089/Images/ab181089-8-benefits-of-recombinant-antibodies.png)
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] (ab181089)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (8)
ab181089 has been referenced in 8 publications.
- Khaliq M et al. Epigenetic modulation reveals differentiation state specificity of oncogene addiction. Nat Commun 12:1536 (2021). PubMed: 33750776
- Yang Y et al. MicroRNA let-7i Inhibits Histone Lysine Demethylase KDM5B to Halt Esophageal Cancer Progression. Mol Ther Nucleic Acids 22:846-861 (2020). PubMed: 33230480
- Xu LM et al. Overcoming of Radioresistance in Non-small Cell Lung Cancer by microRNA-320a Through HIF1a-Suppression Mediated Methylation of PTEN. Front Cell Dev Biol 8:553733 (2020). PubMed: 33304897
- Hao T et al. MicroRNA-363-3p/p21(Cip1/Waf1) axis is regulated by HIF-2a in mediating stemness of melanoma cells. Neoplasma 66:427-436 (2019). PubMed: 30784290
- Li H et al. SNHG1 promotes malignant biological behaviors of glioma cells via microRNA-154-5p/miR-376b-3p- FOXP2- KDM5B participating positive feedback loop. J Exp Clin Cancer Res 38:59 (2019). PubMed: 30728054
- Zhou C et al. H3K27me3 is an epigenetic barrier while KDM6A overexpression improves nuclear reprogramming efficiency. FASEB J 33:4638-4652 (2019). PubMed: 30673507
- Lebrun N et al. Novel KDM5B splice variants identified in patients with developmental disorders: Functional consequences. Gene 679:305-313 (2018). PubMed: 30217758
- Gong J et al. Increased Expression of Lysine-Specific Demethylase 5B (KDM5B) Promotes Tumor Cell Growth in Hep3B Cells and is an Independent Prognostic Factor in Patients with Hepatocellular Carcinoma. Med Sci Monit 24:7586-7594 (2018). PubMed: 30353907