Recombinant Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)
![Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)](https://www.abcam.com/ps/products/232/ab232590/Images/ab232590-308788-anti-kdm5b-plu1-jarid1b-antibody-epr12704-western-blot.jpg "Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free
See all KDM5B / PLU1 / Jarid1B primary antibodies -
Description
Rabbit monoclonal [EPR12704] to KDM5B / PLU1 / Jarid1B - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HAP1 whole cell lysate; HeLa whole cell lysate.
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General notes
ab232590 is the carrier-free version of ab181089.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12704 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). |
Target
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Function
Histone demethylase that demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9' or H3 'Lys-27'. Demethylates trimethylated, dimethylated and monomethylated H3 'Lys-4'. Acts as a transcriptional corepressor for FOXG1B and PAX9. Favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, may act as a tumor suppressor for melanoma. -
Tissue specificity
Ubiquitously expressed, with highest levels in testis. Down-regulated in melanoma and glioblastoma. Up-regulated in breast cancer (at protein level). -
Sequence similarities
Belongs to the JARID1 histone demethylase family.
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain.
Contains 3 PHD-type zinc fingers. -
Domain
Both the JmjC domain and the JmjN domain are required for enzymatic activity.
The 2 first PHD-type zinc finger domains are required for transcription repression activity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10765 Human
- Entrez Gene: 75605 Mouse
- Entrez Gene: 304809 Rat
- Omim: 605393 Human
- SwissProt: Q9UGL1 Human
- SwissProt: Q80Y84 Mouse
- Unigene: 443650 Human
- Unigene: 28995 Mouse
see all -
Alternative names
- Cancer/testis antigen 31 antibody
- CT31 antibody
- Histone demethylase JARID1B antibody
see all
Images
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Western blot - Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: KDM5B knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab181089 observed at 170 kDa. Red - loading control, ab18058, observed at 120 kDa.
ab181089 was shown to recognize KDM5B in wild-type HAP1 cells as signal was lost at the expected MW in KDM5B knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and KDM5B knockout samples were subjected to SDS-PAGE. ab181089 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1.9 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181089).
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![Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)](https://www.abcam.com/ps/products/232/ab232590/Images/ab232590-3-benefits-of-recombinant-antibodies.png)
Anti-KDM5B / PLU1 / Jarid1B antibody [EPR12704] - BSA and Azide free (ab232590)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab232590 has not yet been referenced specifically in any publications.
