Recombinant Anti-Keap1 antibody [EPR22664-26] (ab227828)
![Western blot - Anti-Keap1 antibody [EPR22664-26] (ab227828)](https://www.abcam.com/ps/products/227/ab227828/Images/ab227828-458991-anti-keap1-antibody-western-blot-lung-hap1-hepg2-liver-human-mouse.jpg "Western blot - Anti-Keap1 antibody [EPR22664-26] (ab227828)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22664-26] to Keap1
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Keap1 antibody [EPR22664-26]
See all Keap1 primary antibodies -
Description
Rabbit monoclonal [EPR22664-26] to Keap1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung carcinoma tissue; mouse kidney tissue; WB: human lung tissue, wild type HAP1 whole cell lysate, HepG2 whole cell lysate and mouse E14.5 liver tissue lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22664-26 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
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| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 70 kDa. |
Target
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Function
Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. -
Tissue specificity
Broadly expressed, with highest levels in skeletal muscle. -
Sequence similarities
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats. -
Domain
The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5. -
Post-translational
modificationsUbiquitinated and subject to proteasomal degradation. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9817 Human
- Entrez Gene: 50868 Mouse
- Omim: 606016 Human
- SwissProt: Q14145 Human
- SwissProt: Q9Z2X8 Mouse
- Unigene: 465870 Human
- Unigene: 248266 Mouse
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Alternative names
- Cytosolic inhibitor of Nrf2 antibody
- INrf 2 antibody
- INrf2 antibody
see all
Images
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Western blot - Anti-Keap1 antibody [EPR22664-26] (ab227828)All lanes : Anti-Keap1 antibody [EPR22664-26] (ab227828) at 1/1000 dilution
Lane 1 : Human lung tissue lysate
Lane 2 : Wild-type HAP1 whole cell lysate
Lane 3 : Keap1 knockout HAP1 whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 5 : Mouse E14.5 liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 70 kDa
Exposure time: 3 minutesBlocking/diluting buffer: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] (ab227828)](https://www.abcam.com/ps/products/227/ab227828/Images/ab227828-2-anti-keap1-antibody-epr22264-26-immunohistochemistry-kidney-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] (ab227828)Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Keap1 with ab227828 at 1/500 dilution (0.97 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on kidney tubules of mouse kidney (PMID: 28324005) is observed. The section was incubated with ab227828 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] (ab227828)](https://www.abcam.com/ps/products/227/ab227828/Images/ab227828-1-anti-keap1-antibody-epr22264-26-immunohistochemistry-lung-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keap1 antibody [EPR22664-26] (ab227828)Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Keap1 with ab227828 at 1/500 dilution (0.97 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tumor cells of human lung carcinoma (PMID: 20534738) is observed. The section was incubated with ab227828 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Anti-Keap1 antibody [EPR22664-26] (ab227828)](https://www.abcam.com/ps/products/227/ab227828/Images/ab227828-4-benefits-of-recombinant-antibodies.png)
Anti-Keap1 antibody [EPR22664-26] (ab227828)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab227828 has been referenced in 5 publications.
- Zhang M et al. Long non-coding RNA MALAT1 plays a protective role in bronchopulmonary dysplasia via the inhibition of apoptosis and interaction with the Keap1/Nrf2 signal pathway. Transl Pediatr 10:265-275 (2021). PubMed: 33708512
- Liao Y et al. Suppressive role of microRNA-130b-3p in ferroptosis in melanoma cells correlates with DKK1 inhibition and Nrf2-HO-1 pathway activation. Hum Cell 34:1532-1544 (2021). PubMed: 34117611
- Chen X et al. Long non-coding RNA XIST inhibits osteoblast differentiation and promotes osteoporosis via Nrf2 hyperactivation by targeting CUL3. Int J Mol Med 48:N/A (2021). PubMed: 34036379
- Gao H et al. Inhibition of Kelch-like epichlorohydrin-related protein 1 promotes the progression and drug resistance of lung adenocarcinoma. PeerJ 9:e11908 (2021). PubMed: 34466284
- Wang H et al. Inhibition of LONP1 protects against erastin-induced ferroptosis in Pancreatic ductal adenocarcinoma PANC1 cells. Biochem Biophys Res Commun 522:1063-1068 (2020). PubMed: 31822343