Anti-KIF18B antibody (ab168812)
Key features and details
- Rabbit polyclonal to KIF18B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KIF18B antibody
See all KIF18B primary antibodies -
Description
Rabbit polyclonal to KIF18B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey, Macaque monkey, Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human KIF18B aa 800-900.
Database link: Q86Y91 -
Positive control
- WB: HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
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| Notes |
|---|
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WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa. |
Target
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Function
In complex with KIF2C, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Its major role may be to transport KIF2C and/or MAPRE1 along microtubules. -
Tissue specificity
Shows a prominent expression in the amygdala. -
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
Contains 1 kinesin motor domain. -
Developmental stage
Regulated in a cell cycle-dependent manner. Not expressed in the G1 phase. In G2, sequestered in the nucleus. Maximal levels seen at late G2/M phase and early prometaphase. Degraded at the metaphase-anaphase transition (at protein level). -
Cellular localization
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton. Present predominantly in the nucleus and to a lesser extent in the cytoplasm of interphase cells. During mitosis, found to be closely associated with astral microtubule plus ends emanating from the spindle pole during prometaphase and metaphase. - Information by UniProt
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Database links
- Entrez Gene: 146909 Human
- Omim: 614570 Human
- SwissProt: Q86Y91 Human
- Unigene: 135094 Human
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Alternative names
- KI18B_HUMAN antibody
- KIF18B antibody
- Kinesin family member 18B antibody
- Kinesin-like protein KIF18B antibody
Images
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Western blot - Anti-KIF18B antibody (ab168812)All lanes : Anti-KIF18B antibody (ab168812) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 3 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab168812 has been referenced in 5 publications.
- Wu YP et al. Kinesin family member 18B regulates the proliferation and invasion of human prostate cancer cells. Cell Death Dis 12:302 (2021). PubMed: 33753726
- Li B et al. KIF18B promotes the proliferation of pancreatic ductal adenocarcinoma via activating the expression of CDCA8. J Cell Physiol 235:4227-4238 (2020). PubMed: 31875977
- Li X et al. Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma. Am J Transl Res 12:1614-1639 (2020). PubMed: 32509165
- Gao T et al. KIF18B promotes tumor progression in osteosarcoma by activating ß-catenin. Cancer Biol Med 17:371-386 (2020). PubMed: 32587775
- Liu W et al. Silencing KIF18B enhances radiosensitivity: identification of a promising therapeutic target in sarcoma. EBioMedicine 61:103056 (2020). PubMed: 33038765