Anti-KIF3A antibody (ab11259)
Key features and details
- Rabbit polyclonal to KIF3A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF3A antibody
See all KIF3A primary antibodies -
Description
Rabbit polyclonal to KIF3A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB | (9) |
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide. |
| IHC-P |
1/100.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide. |
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IHC-P
1/100. |
Target
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Function
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin II subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 11127 Human
- Entrez Gene: 16568 Mouse
- Entrez Gene: 84392 Rat
- Omim: 604683 Human
- SwissProt: Q9Y496 Human
- SwissProt: P28741 Mouse
- Unigene: 43670 Human
- Unigene: 88694 Mouse
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Alternative names
- KIF 3 antibody
- KIF 3A antibody
- KIF3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF3A antibody (ab11259)
Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF3A antibody (ab11259)Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF3A antibody (ab11259)Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIF3A antibody (ab11259)Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab11259 has been referenced in 36 publications.
- Zhou Q et al. Kinesin family member 3A stimulates cell proliferation, migration, and invasion of bladder cancer cells in vitro and in vivo. FEBS Open Bio 11:1487-1496 (2021). PubMed: 31774623
- Cullen CL et al. Kif3a deletion prevents primary cilia assembly on oligodendrocyte progenitor cells, reduces oligodendrogenesis and impairs fine motor function. Glia 69:1184-1203 (2021). PubMed: 33368703
- Wang W et al. Suppression of KIF3A inhibits triple negative breast cancer growth and metastasis by repressing Rb-E2F signaling and epithelial-mesenchymal transition. Cancer Sci 111:1422-1434 (2020). PubMed: 32011034
- Li S et al. Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex. Theranostics 10:1719-1732 (2020). PubMed: 32042332
- Mc Fie M et al. Ciliary proteins specify the cell inflammatory response by tuning NF?B signalling, independently of primary cilia. J Cell Sci 133:N/A (2020). PubMed: 32503942