Anti-KIF3A antibody (ab11259)
Key features and details
- Rabbit polyclonal to KIF3A
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Overview
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Product name
Anti-KIF3A antibody
See all KIF3A primary antibodies -
Description
Rabbit polyclonal to KIF3A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human -
Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 682-701 of Mouse KIF3A. This sequence is identical in human.
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General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11259 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (9) |
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa.
This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide. |
IHC-P |
1/100.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 80 kDa. This concentration is determined by blotting using a rat brain homogenate and a whole cell extract of rat pheochromocytoma PC12 cell line. Staining of KIF3A is specifically inhibited by the KIF3A immunizing peptide. |
IHC-P
1/100. |
Target
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Function
Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. -
Sequence similarities
Belongs to the kinesin-like protein family. Kinesin II subfamily.
Contains 1 kinesin-motor domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 11127 Human
- Entrez Gene: 16568 Mouse
- Entrez Gene: 84392 Rat
- Omim: 604683 Human
- SwissProt: Q9Y496 Human
- SwissProt: P28741 Mouse
- Unigene: 43670 Human
- Unigene: 88694 Mouse
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Alternative names
- KIF 3 antibody
- KIF 3A antibody
- KIF3 antibody
see all
Images
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Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.
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Paraffin-embedded rat brain tissue stained for KIF3A with ab11259 at 0.1 μg/mL in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for KIF3A with ab11259 at 0.4 μg/mL in immunohistochemical analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab11259 has been referenced in 41 publications.
- Gifford V et al. Coordination of two kinesin superfamily motor proteins, KIF3A and KIF13A, is essential for pericellular matrix degradation by membrane-type 1 matrix metalloproteinase (MT1-MMP) in cancer cells. Matrix Biol 107:1-23 (2022). PubMed: 35122963
- Wakatsuki S et al. Small noncoding vault RNA modulates synapse formation by amplifying MAPK signaling. J Cell Biol 220:N/A (2021). PubMed: 33439240
- Swarnkar S et al. Molecular motor protein KIF5C mediates structural plasticity and long-term memory by constraining local translation. Cell Rep 36:109369 (2021). PubMed: 34260917
- Ebke LA et al. Photoreceptor Compartment-Specific TULP1 Interactomes. Int J Mol Sci 22:N/A (2021). PubMed: 34360830
- Zhou Q et al. Kinesin family member 3A stimulates cell proliferation, migration, and invasion of bladder cancer cells in vitro and in vivo. FEBS Open Bio 11:1487-1496 (2021). PubMed: 31774623