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Recombinant

Recombinant Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)

Submit a review Q&A (2)References (7)
Western blot - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
  • Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
  • Flow Cytometry - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [S112] to Kir2.1/KCNJ2
  • Suitable for: WB, ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human, African green monkey

Overview

  • Product name

    Anti-Kir2.1/KCNJ2 antibody [S112]
    See all Kir2.1/KCNJ2 primary antibodies

  • Description

    Mouse monoclonal [S112] to Kir2.1/KCNJ2

  • Host species

    Mouse

  • Specificity

    No cross reactivity against Kir2.2, or Kir2.3

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human, African green monkey

  • Immunogen

    This product was produced with the following immunogens:
    Recombinant fragment within Mouse Kir2.1/KCNJ2 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P35561

    Fusion protein corresponding to Mouse Kir2.1/KCNJ2 aa 189-428.
    Database link: P35561

  • Positive control

    • Rat brain normal tissue lysate - membrane extract (ab29473) can be used as a positive control in WB. Flow Cyt: SH-SY5Y cells. IHC-P: Human hippocampal tissue. ICC/IF: SK-N-SH cells.

  • General notes

    The clone number has been updated from S112B-14 to N112B/14, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    S112

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab85492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.

If bands are weak, use lysate without boiling, heat at 37°C for 15 minutes.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.

If bands are weak, use lysate without boiling, heat at 37°C for 15 minutes.
ICC/IF
Use a concentration of 1 - 10 µg/ml.
IHC-P
Use at an assay dependent concentration.
Flow Cyt
Use 1-2µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium or cesium.

  • Tissue specificity

    Heart, brain, placenta, lung, skeletal muscle, and kidney. Diffusely distributed throughout the brain.

  • Involvement in disease

    Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7) [MIM:170390]; also called Andersen syndrome or Andersen cardiodysrhythmic periodic paralysis. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. LQT7 manifests itself as a clinical triad consisting of potassium-sensitive periodic paralysis, ventricular ectopy and dysmorphic features.
    Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3) [MIM:609622]. Short QT syndromes are heart disorders characterized by idiopathic persistently and uniformly short QT interval on ECG in the absence of structural heart disease in affected individuals. They cause syncope and sudden death. SQT3 has a unique ECG phenotype characterized by asymmetrical T waves.

  • Sequence similarities

    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ2 subfamily.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cardiac inward rectifier potassium channel antibody
      • HHBIRK 1 antibody
      • HHBIRK1 antibody
      • HHIRK 1 antibody
      • HHIRK1 antibody
      • HIRK 1 antibody
      • hIRK1 antibody
      • Inward rectifier K antibody
      • Inward rectifier K(+) channel Kir2.1 antibody
      • Inward rectifier potassium channel 2 antibody
      • inwardly rectifying subfamily J member 2 antibody
      • IRK 1 antibody
      • IRK-1 antibody
      • IRK1 antibody
      • IRK2_HUMAN antibody
      • KCNJ2 antibody
      • KIR2.1 antibody
      • LQT 7 antibody
      • LQT7 antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 2 antibody
      • Potassium inwardly rectifying channel J2 antibody
      • Potassium inwardly rectifying channel subfamily J member 2 antibody
      • SQT 3 antibody
      • SQT3 antibody
      see all

    Images

    • Western blot - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
      Western blot - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
      Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492) at 1/1000 dilution + Monkey COS transient cell lysate at 15 µg with 1.5% BSA for 30 minutes at RT

      Secondary
      Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

      Predicted band size: 48 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)

      ab85492 staining Kir2.1/KCNJ2 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded).

      Incubated with primary antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

    • Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
      Immunocytochemistry/ Immunofluorescence - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)

      ICC/IF image of ab85492 stained SK-N-SH (Human neuroblastoma cell line) cells.

      The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85492, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)

      ab85492 staining Kir2.1/KCNJ2 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).

      Incubated with primary antibody at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

    • Flow Cytometry - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)
      Flow Cytometry - Anti-Kir2.1/KCNJ2 antibody [S112] (ab85492)

      Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab85492 (red line).

      The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85492, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween for 20 minutes used under the same conditions.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (7)

    Publishing research using ab85492? Please let us know so that we can cite the reference in this datasheet.

    ab85492 has been referenced in 7 publications.

    • Fan L  et al. Effects of Ruanmailing in Blocking Early Stages of Atherosclerosis by TNF-α Regulation via Kir2.1. Evid Based Complement Alternat Med 2022:2836880 (2022). PubMed: 35432558
    • Chang C  et al. Glycogen synthase kinase 3 beta inhibitor SB216763 improves Kir2.1 expression after myocardia infraction in rats. J Interv Card Electrophysiol N/A:N/A (2021). PubMed: 33611692
    • Shi Y  et al. Kir2.1 Channel Regulation of Glycinergic Transmission Selectively Contributes to Dynamic Mechanical Allodynia in a Mouse Model of Spared Nerve Injury. Neurosci Bull 35:301-314 (2019). PubMed: 30203408
    • Binda A  et al. A Novel KCNJ2 Mutation Identified in an Autistic Proband Affects the Single Channel Properties of Kir2.1. Front Cell Neurosci 12:76 (2018). PubMed: 29615871
    • Ji CD  et al. Kir2.1 Interaction with Stk38 Promotes Invasion and Metastasis of Human Gastric Cancer by Enhancing MEKK2-MEK1/2-ERK1/2 Signaling. Cancer Res 78:3041-3053 (2018). PubMed: 29549164
    • Kamikawa A  et al. Decrease in an Inwardly Rectifying Potassium Conductance in Mouse Mammary Secretory Cells after Forced Weaning. PLoS One 10:e0141131 (2015). WB ; Mouse . PubMed: 26484867
    • Goldoni D  et al. A novel dual-fluorescence strategy for functionally validating microRNA targets in 3-prime untranslated regions: regulation of the inward rectifier potassium channel Kir2.1 by miR-212. Biochem J : (2012). WB ; Human . PubMed: 22880819

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Hi,

    I'm interested in seeing whether your anti-kir2.1 antibodies will cross-react with the zebrafish kir2.1 protein. However, for the 6 anti-kir2.1 antibodies that you stock there are no details regarding the region the antibodies are targeting, and therefore I can't be sure which antibody is the best suited for my needs. I've included the amino acid sequence of the zebrafish kir2.1 in this email in hopes that you would be able to tell me which antibody target shares the highest homology with it and therefore may be able to cross-react with the zebrafish protein.

    zebrafish kir2.1
    MGSVRANRYSIVSSEEDGMKLATAAVPNGYGNGKSKVHTRHQTQSRFVKKDGHCNVQFIN
    VSEKSQRYLADIFTTCVDIRWRWMFVIFCLAFLLSWLFFGCIFWLVAIFHGDLENNGHKC
    VSNVSSFTAAFLFSIETQTTIGYGYRYVTDECPIAVFMVVFQSIVGCIIDAFIIGAVMAK
    MAKPKKRNETLVFSHNATVAMRDNKLCLMWRVGNLRKSHLVEAHVRAQLLRSRTTAEREF
    IPLDQMDIDVGFDSGIDRIFLVSPITIVHEIDEDSPFYDMSKQEMENSDFEIVVILEGMV
    EATAMTTQCRSSYVANEILWAHRFEPVLFEEKNYYKVDYSRFHKTYEVPSTPLCSARDLA
    EKKYIISNSNSFCYENEVALSNKEEKEEGHGDSLGPGGTNTETSSDSEHSQATVPLEPRP
    LRRESEI
    Kind regards,

    Read More
    Answer

    Thank you for contacting us.
    The immunogen of ab80969 shares 85% homology with Zebrafish so it is more likely to cross-react. Once you decide to buy the antibody I would like to inform you about the Abreview 100% discount programme. Please click the link
    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
    We are also giving away free protein ladder with primary orders. Please check
    https://www.abcam.com/index.html?pageconfig=resource&rid=14968
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Hi Technical,



    We have a customer who is interested in the following antibodies;



    ab85492, ab109750, ab65796, ab80969, ab45390, ab71574.



    Their question is as follows;



    I'm interested in seeing whether your anti-kir2.1 antibodies will cross-react with the zebrafish kir2.1 protein. However, for the 6 anti-kir2.1 antibodies that you stock there are no details regarding the region the antibodies are targeting, and therefore I can't be sure which antibody is the best suited for my needs. I've included the amino acid sequence of the zebrafish kir2.1 in this email in hopes that you would be able to tell me which antibody target shares the highest homology with it and therefore may be able to cross-react with the zebrafish protein.



    zebrafish kir2.1



    MGSVRANRYSIVSSEEDGMKLATAAVPNGYGNGKSKVHTRHQTQSRFVKKDGHCNVQFIN

    VSEKSQRYLADIFTTCVDIRWRWMFVIFCLAFLLSWLFFGCIFWLVAIFHGDLENNGHKC

    VSNVSSFTAAFLFSIETQTTIGYGYRYVTDECPIAVFMVVFQSIVGCIIDAFIIGAVMAK

    MAKPKKRNETLVFSHNATVAMRDNKLCLMWRVGNLRKSHLVEAHVRAQLLRSRTTAEREF

    IPLDQMDIDVGFDSGIDRIFLVSPITIVHEIDEDSPFYDMSKQEMENSDFEIVVILEGMV

    EATAMTTQCRSSYVANEILWAHRFEPVLFEEKNYYKVDYSRFHKTYEVPSTPLCSARDLA

    EKKYIISNSNSFCYENEVALSNKEEKEEGHGDSLGPGGTNTETSSDSEHSQATVPLEPRP

    LRRESEI



    According to the datasheet, they have not been tested with Zebrafish, so I would assume that you can’t confirm for them, but it is best to check. I will also mention to them of Abcam’s testing discount.



    Looking forward to your response.





    Kind regards,

    Read More
    Answer

    Thank youand your customer very much for your interest in our antibodies.

    I checked all immunogens and homology to zebrafish (sequnec that your customer provided)of the Kir2.1 antibodies we offer at the moment.

    Only one antibody shows a sufficiently high homology to expect cross-reactivity: ab71574



    Therefore, I can offer a discount off a future purchase if your customerbuys ab71574 now, tests it inzebrafish and submits feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your customer'sfeedback. The discount would be to the value of ab71574 and can be used to order any product.

    If your customer is interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that your customer ( please include theire-mail address)would like to proceed and test ab71574 in zebrafish. I will then send a discount code. This code must be issued before purchasing ab71574 so please wait for my reply before ordering.

    2. Purchase ab71574 either by phone, fax, or online (www.abcam.com).

    3. Test it in zebrafish.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your customer's new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab71574turns out to be unsuitable for zebrafish, your customerwill still receive the discount ontheir next purchase after your Abreview has been submitted.

    Please let me know if you or your customer have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com