Recombinant Anti-KLF10 antibody [EPR12102(2)] (ab184182)
![Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182)](https://www.abcam.com/ps/products/184/ab184182/Images/ab184182-216459-anti-klf10-antibody-epr121022-western-blot.jpg "Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12102(2)] to KLF10
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-KLF10 antibody [EPR12102(2)]
See all KLF10 primary antibodies -
Description
Rabbit monoclonal [EPR12102(2)] to KLF10 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa, K652 and HepG2 whole cell lysate (ab7900).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12102(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation. -
Sequence similarities
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7071 Human
- Entrez Gene: 21847 Mouse
- Entrez Gene: 81813 Rat
- Omim: 601878 Human
- SwissProt: Q13118 Human
- SwissProt: O89091 Mouse
- SwissProt: O08876 Rat
- Unigene: 435001 Human
see all -
Alternative names
- Early growth response, alpha antibody
- Early growth response, alpha like antibody
- EGR A antibody
see all
Images
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Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182)All lanes : Anti-KLF10 antibody [EPR12102(2)] (ab184182) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182)](https://www.abcam.com/ps/products/184/ab184182/Images/ab184182-216458-anti-klf10-antibody-epr121022-western-blot.jpg)
Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182)All lanes : Anti-KLF10 antibody [EPR12102(2)] (ab184182) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K652 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Anti-KLF10 antibody [EPR12102(2)] (ab184182)](https://www.abcam.com/ps/products/184/ab184182/Images/ab184182-3-benefits-of-recombinant-antibodies.png)
Anti-KLF10 antibody [EPR12102(2)] (ab184182)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab184182 has been referenced in 2 publications.
- Zou Z et al. MicroRNA-126 engineered muscle-derived stem cells attenuates cavernosa injury-induced erectile dysfunction in rats. Aging (Albany NY) 13:14399-14415 (2021). PubMed: 34031263
- Mishra VK et al. Krüppel-like Transcription Factor KLF10 Suppresses TGFß-Induced Epithelial-to-Mesenchymal Transition via a Negative Feedback Mechanism. Cancer Res 77:2387-2400 (2017). PubMed: 28249899