Anti-KLF2 antibody [OTI5F1] (ab236507)
![Western blot - Anti-KLF2 antibody [OTI5F1] (ab236507)](https://www.abcam.com/ps/products/236/ab236507/Images/ab236507-312886-anti-klf2-antibody-oti5f1-western-blot.jpg "Western blot - Anti-KLF2 antibody [OTI5F1] (ab236507)")
Key features and details
- Mouse monoclonal [OTI5F1] to KLF2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-KLF2 antibody [OTI5F1]
See all KLF2 primary antibodies -
Description
Mouse monoclonal [OTI5F1] to KLF2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human KLF2 aa 1 to the C-terminus. (NP_057354) produced in E.coli.
Database link: Q9Y5W3 -
Positive control
- WB: HEK-293T transfected with pCMV6-ENTRY KLF2 whole cell lysate.
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General notes
Clone OTI5F1 (formerly 5F1).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI5F1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236507 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 37 kDa.
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| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 37 kDa. |
Target
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Function
Binds to the CACCC box in the beta-globin gene promoter and activates transcription. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsUbiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10365 Human
- Omim: 602016 Human
- SwissProt: Q9Y5W3 Human
- Unigene: 726356 Human
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Alternative names
- KLF 2 antibody
- Klf2 antibody
- KLF2_HUMAN antibody
see all
Images
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Western blot - Anti-KLF2 antibody [OTI5F1] (ab236507)All lanes : Anti-KLF2 antibody [OTI5F1] (ab236507) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY KLF2 whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 37 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab236507 has been referenced in 1 publication.
- Li W et al. Azilsartan ameliorates ox-LDL-induced endothelial dysfunction via promoting the expression of KLF2. Aging (Albany NY) 13:12996-13005 (2021). PubMed: 33946046