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    products/primary-antibodies/klf2-antibody-oti5f1-ab236507.pdf

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Cancer Invasion/microenvironment Angiogenesis Angiogenic inhibitory factors
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Anti-KLF2 antibody [OTI5F1] (ab236507)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

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Western blot - Anti-KLF2 antibody [OTI5F1] (ab236507)

    Key features and details

    • Mouse monoclonal [OTI5F1] to KLF2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-KLF2 antibody [OTI5F1]
      See all KLF2 primary antibodies
    • Description

      Mouse monoclonal [OTI5F1] to KLF2
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Recombinant full length protein corresponding to Human KLF2 aa 1 to the C-terminus. (NP_057354) produced in E.coli.
      Database link: Q9Y5W3

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • WB: HEK-293T transfected with pCMV6-ENTRY KLF2 whole cell lysate.
    • General notes

      Clone OTI5F1 (formerly 5F1).

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 1% BSA, 50% Glycerol
    • Concentration information loading...
    • Purity

      Affinity purified
    • Purification notes

      Purified from cell culture supernatant.
    • Clonality

      Monoclonal
    • Clone number

      OTI5F1
    • Isotype

      IgG1
    • Research areas

      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • Angiogenic inhibitory factors
      • Cardiovascular
      • Vasculature
      • Endothelium
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Krueppel like factor
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Krueppel like factor

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human KLF2 protein (ab160964)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab236507 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/2000. Predicted molecular weight: 37 kDa.
    Notes
    WB
    1/2000. Predicted molecular weight: 37 kDa.

    Target

    • Function

      Binds to the CACCC box in the beta-globin gene promoter and activates transcription.
    • Sequence similarities

      Belongs to the krueppel C2H2-type zinc-finger protein family.
      Contains 3 C2H2-type zinc fingers.
    • Post-translational
      modifications

      Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9Y5W3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10365 Human
      • Omim: 602016 Human
      • SwissProt: Q9Y5W3 Human
      • Unigene: 726356 Human
      • Alternative names

        • KLF 2 antibody
        • Klf2 antibody
        • KLF2_HUMAN antibody
        • Krueppel-like factor 2 antibody
        • Kruppel like factor 2 antibody
        • Kruppel like factor antibody
        • Kruppel like factor LKLF antibody
        • Kruppel-like factor 2 (lung) antibody
        • Lklf antibody
        • Lung krueppel like factor antibody
        • Lung krueppel-like factor antibody
        • Lung Kruppel like zinc finger transcription factor antibody
        see all

      Images

      • Western blot - Anti-KLF2 antibody [OTI5F1] (ab236507)
        Western blot - Anti-KLF2 antibody [OTI5F1] (ab236507)
        All lanes : Anti-KLF2 antibody [OTI5F1] (ab236507) at 1/2000 dilution

        Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control whole cell lysate
        Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY KLF2 whole cell lysate

        Lysates/proteins at 5 µg per lane.

        Predicted band size: 37 kDa

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab236507? Please let us know so that we can cite the reference in this datasheet.

      ab236507 has been referenced in 1 publication.

      • Li W  et al. Azilsartan ameliorates ox-LDL-induced endothelial dysfunction via promoting the expression of KLF2. Aging (Albany NY) 13:12996-13005 (2021). PubMed: 33946046

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      IHC - Wholemount abreview for Anti-KLF2 antibody [OTI5F1]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Mouse Embryo (E3.75 mouse blastocyst)
      Specification
      E3.75 mouse blastocyst
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MR. Vidur Garg

      Verified customer

      Submitted Oct 23 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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