Anti-KLF3 antibody (ab154531)
Key features and details
- Rabbit polyclonal to KLF3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-KLF3 antibody -
Description
Rabbit polyclonal to KLF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
-
Immunogen
Recombinant fragment, corresponding to a region within amino acids 153-334 of Human KLF3 (UniProt P57682).
-
Positive control
- A549 and HCT116 cell lysates; SCC4 xenograft tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154531 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 39 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 39 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
-
Function
Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsSumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 530358 Cow
- Entrez Gene: 488836 Human
- Entrez Gene: 51274 Human
- Entrez Gene: 16599 Mouse
- Entrez Gene: 100174960 Pig
- Entrez Gene: 114845 Rat
- Omim: 609392 Human
- SwissProt: P57682 Human
see all -
Alternative names
- Basic krueppel-like factor antibody
- Basic Kruppel like factor antibody
- BKLF3 antibody
see all
Images
-
Western blot - Anti-KLF3 antibody (ab154531)Anti-KLF3 antibody (ab154531) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 39 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF3 antibody (ab154531)Immunohistochemical analysis of paraffin-embedded SCC4 xenograft tissue labeling KLF3 with ab154531 at 1/100 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab154531 has been referenced in 4 publications.
- Zhang J et al. Long non-coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA-324-5p and upregulating KCTD20 expression. Oncol Rep 46:N/A (2021). PubMed: 33982764
- Liu JQ et al. lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition. Mol Ther Nucleic Acids 20:231-241 (2020). PubMed: 32193151
- Wu S et al. Circ_0084043 promotes cell proliferation and glycolysis but blocks cell apoptosis in melanoma via circ_0084043-miR-31-KLF3 axis. Open Life Sci 15:774-786 (2020). PubMed: 33817265
- Wang X et al. RNA sequencing analysis reveals protective role of kruppel-like factor 3 in colorectal cancer. Oncotarget 8:21984-21993 (2017). IHC-P ; Human . PubMed: 28423541