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    products/primary-antibodies/klf4-antibody-ab129473.pdf

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Epigenetics and Nuclear Signaling Transcription Other factors
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Anti-KLF4 antibody (ab129473)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (24)

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Western blot - Anti-KLF4 antibody (ab129473)
  • Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (ab129473)
  • Western blot - Anti-KLF4 antibody (ab129473)

Key features and details

  • Rabbit polyclonal to KLF4
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-KLF4 antibody [EPR20753-25] (ab214666)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-KLF4 antibody
    See all KLF4 primary antibodies
  • Description

    Rabbit polyclonal to KLF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Mouse KLF4 aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q60793

  • Positive control

    • WB: Mouse ES-E14TG2a whole cell lysate, Mouse embryonic stem cell lysate, F9 whole cell lysate, LoVo whole cell lysate, HEK-293 whole cell lysate, HT29 whole cell lysate ICC/IF: mES cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Embryonic Stem Cells
    • Intracellular
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Other
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Krueppel like factor
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Krueppel like factor
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Surface molecules
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human KLF4 protein (ab169841)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
Use a concentration of 1 µg/ml.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
Notes
ICC/IF
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).

Target

  • Function

    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.
  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O43474 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9314 Human
    • Entrez Gene: 16600 Mouse
    • Entrez Gene: 114505 Rat
    • Omim: 602253 Human
    • SwissProt: O43474 Human
    • SwissProt: Q60793 Mouse
    • Unigene: 376206 Human
    • Unigene: 4325 Mouse
    • Alternative names

      • Endothelial Kruppel like zinc finger protein antibody
      • Epithelial zinc finger protein EZF antibody
      • EZF antibody
      • GKLF antibody
      • gut Kruppel-like factor antibody
      • Gut-enriched krueppel-like factor antibody
      • KLF antibody
      • KLF4 antibody
      • KLF4_HUMAN antibody
      • Krueppel-like factor 4 antibody
      • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
      • Kruppel like factor 4 (gut) antibody
      see all

    Images

    • Western blot - Anti-KLF4 antibody (ab129473)
      Western blot - Anti-KLF4 antibody (ab129473)
      All lanes : Anti-KLF4 antibody (ab129473) at 1 µg/ml

      Lane 1 : Mouse embryonic stem cell lysate
      Lane 2 : F9 whole cell lysate
      Lane 3 : LoVo whole cell lysate
      Lane 4 : HEK-293 whole cell lysate
      Lane 5 : HT29 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

      Predicted band size: 54 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Gel type: MOPS

      Blocking buffer: 2% BSA block

    • Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (ab129473)
      Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (ab129473)

      ab129473 staining KLF4 in mES cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab129473 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Western blot - Anti-KLF4 antibody (ab129473)
      Western blot - Anti-KLF4 antibody (ab129473)
      Anti-KLF4 antibody (ab129473) at 1 µg/ml + E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 54 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?


      Exposure time: 8 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129473 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (24)

    Publishing research using ab129473? Please let us know so that we can cite the reference in this datasheet.

    ab129473 has been referenced in 24 publications.

    • Cheng Z  et al. KLF4 Exerts Sedative Effects in Pentobarbital-Treated Mice. J Mol Neurosci 71:596-606 (2021). PubMed: 32789565
    • Liu W  et al. miR-140 inhibits osteosarcoma progression by impairing USP22-mediated LSD1 stabilization and promoting p21 expression. Mol Ther Nucleic Acids 24:436-448 (2021). PubMed: 33868787
    • Xu JH  et al. Deletion of Krüppel-like factor-4 promotes axonal regeneration in mammals. Neural Regen Res 16:166-171 (2021). PubMed: 32788472
    • Li C  et al. KLF4 down-regulation resulting from TLR4 promotion of ERK1/2 phosphorylation underpins inflammatory response in sepsis. J Cell Mol Med 25:2013-2024 (2021). PubMed: 33369167
    • Liu SC  et al. Inhibition of Bruton's tyrosine kinase as a therapeutic strategy for chemoresistant oral squamous cell carcinoma and potential suppression of cancer stemness. Oncogenesis 10:20 (2021). PubMed: 33640903
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KLF4 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (primary astrocytes)
    Permeabilization
    No
    Specification
    primary astrocytes
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Acetone
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 10 2018

    Western blot abreview for Anti-KLF4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (mouse embryonic stem cells (46C))
    Specification
    mouse embryonic stem cells (46C)
    Treatment
    transfection with Klf4 expression vector (Flag-tagged Klf4)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 04 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-KLF4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 21°C
    Sample
    Mouse Cell (D3 Mouse Embryonic Stem Cell line)
    Specification
    D3 Mouse Embryonic Stem Cell line
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Joe Segal

    Verified customer

    Submitted Jul 18 2013

    Western blot abreview for Anti-KLF4 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    20000 cells
    Gel Running Conditions
    Reduced Denaturing (gel 10%)
    Sample
    Mouse Cell lysate - whole cell (D3 Mouse Embryonic Stem Cell line)
    Specification
    D3 Mouse Embryonic Stem Cell line
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Joe Segal

    Verified customer

    Submitted Jul 02 2013

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