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RecombinantRabMAb

Recombinant Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

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Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
  • Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
  • Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
  • Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19590] to KLF4 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-KLF4 antibody [EPR19590] - BSA and Azide free
    See all KLF4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19590] to KLF4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NCCIT whole cell lysate. IHC-P: Human stomach, gastric cancer and seminoma tissues. ICC/IF: NCCIT cells. Flow Cyt (intra): NCCIT cells.

  • General notes

    ab222235 is the carrier-free version of ab215036.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

Target

  • Function

    Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 3 C2H2-type zinc fingers.

  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Endothelial Kruppel like zinc finger protein antibody
      • Epithelial zinc finger protein EZF antibody
      • EZF antibody
      • GKLF antibody
      • gut Kruppel-like factor antibody
      • Gut-enriched krueppel-like factor antibody
      • KLF antibody
      • KLF4 antibody
      • KLF4_HUMAN antibody
      • Krueppel-like factor 4 antibody
      • Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) antibody
      • Kruppel like factor 4 (gut) antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling KLF4 with ab215036 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on NCCIT cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215036).

    • Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

      ab215036 (purified) at 1/30 dilution immunoprecipitating KLF4 in NCCIT whole cell lysate.
      Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10 µg
      Lane 2 (+): ab215036 & NCCIT whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab215036 in NCCIT whole cell lysate
      For western blotting, ab215036 at 1/500 dilution (0.117 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/1000 dilution were used.
      Blocking and diluting buffer: 5% NFDM /TBST .
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215036)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

      Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling KLF4 with ab215036 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution. 

      Nuclear staining on human stomach is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215036).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

      Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling KLF4 with ab215036 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      Nuclear staining on tumor cells of human gastric cancer is observed [PMID: 17614846].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215036).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

      Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling KLF4 with ab215036 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      Nuclear staining on tumor cells of human seminoma is observed [PMID: 17614846].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215036).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

      Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling  KLF4 with ab215036 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215036). 

       

       

    • Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
      Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)

    References (0)

    Publishing research using ab222235? Please let us know so that we can cite the reference in this datasheet.

    ab222235 has not yet been referenced specifically in any publications.

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