Anti-KLF5 antibody (ab137676)
Key features and details
- Rabbit polyclonal to KLF5
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KLF5 antibody
See all KLF5 primary antibodies -
Description
Rabbit polyclonal to KLF5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow, Pig
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Immunogen
Recombinant fragment corresponding to Human KLF5 aa 69-346.
Database link: Q13887 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
|
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| WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 51 kDa.
|
| ICC/IF | (1) |
1/100 - 1/1000.
|
| Notes |
|---|
|
IHC-P
1/100 - 1/1000. |
|
WB
1/500 - 1/3000. Predicted molecular weight: 51 kDa. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes. -
Tissue specificity
Expressed only in testis and placenta. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers. -
Post-translational
modificationsUbiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 535702 Cow
- Entrez Gene: 688 Human
- Entrez Gene: 12224 Mouse
- Entrez Gene: 100038005 Pig
- Omim: 602903 Human
- SwissProt: Q13887 Human
- SwissProt: Q9Z0Z7 Mouse
- Unigene: 508234 Human
see all -
Form
KLF5 (also known as basic transcription element-binding protein 2, BTEB2, and intestine enriched krüppel-like factor, IKLF), appears to play a central role in the regulation of intestinal epithelial renewal in both the small intestine and colon. KLF5 also regulates adipocyte differentiation. -
Alternative names
- Basic transcription element binding protein 2 antibody
- Basic transcription element-binding protein 2 antibody
- BTE binding protein 2 antibody
see all
Images
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Western blot - Anti-KLF5 antibody (ab137676)All lanes : Anti-KLF5 antibody (ab137676) at 1/5000 dilution
Lane 1 : Non-transfected 293T whole cell extract
Lane 2 : Transfected 293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342. -
Western blot - Anti-KLF5 antibody (ab137676)All lanes : Anti-KLF5 antibody (ab137676) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab137676)ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500
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Western blot - Anti-KLF5 antibody (ab137676)Anti-KLF5 antibody (ab137676) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 51 kDa
10% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (26)
ab137676 has been referenced in 26 publications.
- Moritake T et al. Krüppel-like factor 5 is upregulated and induces cell proliferation in endometrial cancer. Oncol Lett 21:484 (2021). PubMed: 33968200
- Xiao L et al. Inhibition of HDAC4 by GSK3ß leads to downregulation of KLF5 and ASK1 and prevents the progression of intravertebral disc degeneration. Clin Epigenetics 13:53 (2021). PubMed: 33691773
- Nan S et al. Interfering microRNA-410 attenuates atherosclerosis via the HDAC1/KLF5/IKBa/NF-?B axis. Mol Ther Nucleic Acids 24:646-657 (2021). PubMed: 33981482
- Pratheeshkumar P et al. Prognostic Value and Function of KLF5 in Papillary Thyroid Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 33430300
- Chang Y et al. Metapristone (RU486-derivative) inhibits endometrial cancer cell progress through regulating miR-492/Klf5/Nrf1 axis. Cancer Cell Int 21:29 (2021). PubMed: 33413440