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RecombinantRabMAb

Recombinant Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

Submit a review Submit a question References (4)
Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
  • Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
  • Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
  • Flow Cytometry - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
  • ChIC/CUT&RUN sequencing - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
  • Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP129] to KMT6 / EZH2 - C-terminal
  • Suitable for: WB, IHC-P, Flow Cyt, ChIC/CUT&RUN-seq, IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-KMT6 / EZH2 antibody [SP129] - C-terminal
    See all KMT6 / EZH2 primary antibodies

  • Description

    Rabbit monoclonal [SP129] to KMT6 / EZH2 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ChIC/CUT&RUN-seq, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Monkey

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate (ab150035), NIH/3T3 and HEK-293 cell lysates; Mouse brain tissue lysate. IHC-P: Human prostate cancer tissue. Flow Cyt: HeLa cells. IP: HEK-293 whole cell lysate ChIC/CUT&RUN-seq: K-562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/25. Predicted molecular weight: 85 kDa.

Primary antibody incubation for 1 hour at room temperature.
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.
Flow Cyt
1/100.

Primary antibody incubation for 30 minutes at 4°C.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
IP
1/20.
Notes
WB
1/25. Predicted molecular weight: 85 kDa.

Primary antibody incubation for 1 hour at room temperature.
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.
Flow Cyt
1/100.

Primary antibody incubation for 30 minutes at 4°C.
ChIC/CUT&RUN-seq
Use at an assay dependent concentration.
IP
1/20.

Target

  • Function

    Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes.

  • Tissue specificity

    Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.

  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. EZ subfamily.
    Contains 1 SET domain.

  • Developmental stage

    Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.

  • Post-translational
    modifications

    Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Enhancer of zeste 2 antibody
      • enhancer of zeste 2 polycomb repressive complex 2 subunit antibody
      • Enhancer of zeste homolog 2 (Drosophila) antibody
      • Enhancer of zeste homolog 2 antibody
      • Enhancer of zeste, Drosophila, homolog 2 antibody
      • ENX 1 antibody
      • Enx 1h antibody
      • ENX-1 antibody
      • ENX1 antibody
      • Enx1h antibody
      • EZH 2 antibody
      • EZH1 antibody
      • EZH2 antibody
      • EZH2_HUMAN antibody
      • EZH2b antibody
      • Histone-lysine N-methyltransferase EZH2 antibody
      • KMT 6 antibody
      • KMT6 antibody
      • KMT6A antibody
      • Lysine N-methyltransferase 6 antibody
      • MGC9169 antibody
      • WVS antibody
      • WVS2 antibody
      see all

    Images

    • Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      All lanes : Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 0.316 µg/ml

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) cell lysate
      Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) cell lysate
      Lane 3 : Mouse brain tissue lysate
      Lane 4 : HEK-293 (Human embryonic kidney epithelial cell) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

      Predicted band size: 85 kDa
      Observed band size: 85 kDa



      Blocking/Diluting buffer and concentration: 5% NFDM /TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

      Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for KMT6 / EZH2 using ab227648 at 1/100 dilution in immunohistochemical analysis.

    • Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

      ab227648 (purified) at 1/20 dilution (20 µg/ml) immunoprecipitating KMT6 in HEK-293 whole cell lysate.

      Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab227648 & HEK-293 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab227648 in HEK-293 whole cell lysate

      For western blotting, ab227648 (purified at 1/500 dilution (0.632 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
      Blocking and diluting buffer: 5% NFDM /TBST.

    • Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Western blot - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648) at 1/25 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

      Predicted band size: 85 kDa

    • Flow Cytometry - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Flow Cytometry - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

      Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling KMT6 / EZH2 with ab227648 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

    • ChIC/CUT&RUN sequencing - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      ChIC/CUT&RUN sequencing - Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

      CUT&RUN was performed using the ChIC/CUT&RUN pAG-MNAse ab285373, 2.5 x 10^5 K-562 (Human chronic myelogenous leukemia lymphoblast cell line) cells and 5µg of ab227648 [SP129]. The resulting DNA was sequenced on the NovaSeq 6000 (PE150).

      Additional screenshots of mapped reads can be downloaded here.

    • Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)
      Anti-KMT6 / EZH2 antibody [SP129] - C-terminal (ab227648)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (4)

    Publishing research using ab227648? Please let us know so that we can cite the reference in this datasheet.

    ab227648 has been referenced in 4 publications.

    • Zhang H  et al. m6A methyltransferase METTL3-induced lncRNA SNHG17 promotes lung adenocarcinoma gefitinib resistance by epigenetically repressing LATS2 expression. Cell Death Dis 13:657 (2022). WB ; Human . PubMed: 35902569
    • Huang K & Tang Y SChLAP1 promotes prostate cancer development through interacting with EZH2 to mediate promoter methylation modification of multiple miRNAs of chromosome 5 with a DNMT3a-feedback loop. Cell Death Dis 12:188 (2021). PubMed: 33589600
    • Xiang W  et al. The long non-coding RNA SNHG1 promotes bladder cancer progression by interacting with miR-143-3p and EZH2. J Cell Mol Med 24:11858-11873 (2020). PubMed: 32885590
    • Singla N  et al. Multi-institutional evaluation of the prognostic significance of EZH2 expression in high-grade upper tract urothelial carcinoma. Urol Oncol N/A:N/A (2018). PubMed: 29748098

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com