Recombinant Anti-Kv1.1 potassium channel antibody [K36/15] (ab300664)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-488317-anti-kcna1-antibody-K36-15-ihcp-mouse-hippocampus.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [K36/15] to Kv1.1 potassium channel
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Kv1.1 potassium channel antibody [K36/15]
See all Kv1.1 potassium channel primary antibodies -
Description
Mouse monoclonal [K36/15] to Kv1.1 potassium channel -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse and rat hippocampus, FFPE tissue sections. IHC-Fr: Rat hippocampus, fresh frozen tissues.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
K36/15 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
1/500.
Not Applicable. |
| Notes |
|---|
|
IHC-P
1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/500. Not Applicable. |
Target
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Function
Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. -
Involvement in disease
Defects in KCNA1 are the cause of episodic ataxia type 1 (EA1) [MIM:160120]; also known as paroxysmal or episodic ataxia with myokymia (EAM) or paroxysmal ataxia with neuromyotonia. EA1 is an autosomal dominant disorder characterized by brief episodes of ataxia and dysarthria. Neurological examination during and between the attacks demonstrates spontaneous, repetitive discharges in the distal musculature (myokymia) that arise from peripheral nerve. Nystagmus is absent.
Defects in KCNA1 are the cause of myokymia isolated type 1 (MK1) [MIM:160120]. Myokymia is a condition characterized by spontaneous involuntary contraction of muscle fiber groups that can be observed as vermiform movement of the overlying skin. Electromyography typically shows continuous motor unit activity with spontaneous oligo- and multiplet-discharges of high intraburst frequency (myokymic discharges). Isolated spontaneous muscle twitches occur in many persons and have no grave significance. -
Sequence similarities
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily. -
Domain
The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPalmitoylated on Cys-243; which may be required for membrane targeting. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16485 Mouse
- Entrez Gene: 24520 Rat
- SwissProt: P16388 Mouse
- SwissProt: P10499 Rat
- Unigene: 40424 Mouse
- Unigene: 9769 Rat
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Alternative names
- AEMK antibody
- EA1 antibody
- Episodic ataxia with myokymia antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)
Immunohistochemical analysis of paraffin-embedded mouse hippocampus labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on mouse hippocampus. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody without primary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-488318-anti-kcna1-antibody-K36-15-ihcp-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)Immunohistochemical analysis of paraffin-embedded mouse liver labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: no staining on mouse liver. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody without primary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-488319-anti-kcna1-antibody-K36-15-ihcp-rat-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)Immunohistochemical analysis of paraffin-embedded rat hippocampus labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on rat hippocampus. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody without primary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-488320-anti-kcna1-antibody-K36-15-ihcp-rat-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kcna1 antibody [K36/15] (AB300664)Immunohistochemical analysis of paraffin-embedded rat liver labeling Kcna1 with ab300664 at 1/10000 dilution (0.099 µg/ml) followed by mouse IgG1 antibody (ab125913) for 8 mins and a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Negative control: no staining on rat liver. The section was incubated with ab300664 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody without primary.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
-
![Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-488315-anti-kcna1-antibody-K36-15-ihc-frozen-rat-hippocampus.jpg)
Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh frozen sectioned rat hippocampus tissue labeling Aquaporin 1 with ab300664 at 1/500 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (green). Positive staining on rat hippocampus is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Secondary antibody without adding primary.
No antigen retrieval was carried out.
-
![Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-488316-anti-kcna1-antibody-K36-15-ihc-frozen-rat-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-Kcna1 antibody [K36/15] (AB300664)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized fresh frozen sectioned rat liver tissue labeling Aquaporin 1 with ab300664 at 1/500 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (green). Negative control (PMID: 24309898) - no staining on rat liver is observed. The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Secondary antibody without adding primary.
No antigen retrieval was carried out.
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![Anti-Kcna1 antibody [K36/15] (AB300664)](https://www.abcam.com/ps/products/300/ab300664/Images/AB300664-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Kcna1 antibody [K36/15] (AB300664)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab300664 has not yet been referenced specifically in any publications.