Recombinant Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26384-89] to Kv4.2/KCND2
- Suitable for: IHC-Fr, IP, Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Kv4.2/KCND2 antibody [EPR26384-89]
See all Kv4.2/KCND2 primary antibodies -
Description
Rabbit monoclonal [EPR26384-89] to Kv4.2/KCND2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse cerebellum, Rat cerebellum and His-tagged mouse KCND2 fragment lysates. IHC-P: Mouse and Rat cerebellum. IHC-Fr: Mouse and Rat cerebellum. Flow Cyt (Intra): Mouse and Rat primary neuron cells. IP: Mouse and Rat cerebellum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26384-89 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
1/100.
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IP |
1/30.
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Flow Cyt (Intra) |
1/50.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
WB |
1/1000. Detects a band of approximately 70 kDa.
|
Notes |
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IHC-Fr
1/100. |
IP
1/30. |
Flow Cyt (Intra)
1/50. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 70 kDa. |
Target
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Function
Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits. -
Tissue specificity
Highly expressed throughout the brain. Expression is very low or absent in other tissues. -
Sequence similarities
Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.2/KCND2 sub-subfamily. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylated on serine and threonine residues. -
Cellular localization
Cell membrane. Cell projection > dendrite. Detected in dendrites in cultured hippocampal neurons. Association with KCNIP2 probably enhances cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 16508 Mouse
- Entrez Gene: 65180 Rat
- SwissProt: Q9Z0V2 Mouse
- SwissProt: Q63881 Rat
- Unigene: 425316 Mouse
- Unigene: 87841 Rat
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Alternative names
- KCD2 antibody
- KCND 2 antibody
- KCND2 antibody
see all
Images
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All lanes : Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710) at 1/1000 dilution
Lane 1 : His-tagged mouse KCND1 fragment 10 ng
Lane 2 : His-tagged mouse KCND2 fragment 10 ng
Lane 3 : His-tagged mouse KCND3 fragment 10 ng
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with mouse KCND1 and KCND3.
In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution.
Exposure time: 10 seconds
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All lanes : Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate 20 µg
Lane 2 : Mouse liver tissue lysate 20 µg
Lane 3 : Mouse bladder tissue lysate 20 µg
Lane 4 : Rat cerebellum tissue lysate 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Observed band size: 250,300,70 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: liver(Human Protein Atlas Database), bladder (PMID:12575952).
The molecular weight observed is consistent with what has been described in the literature (PMID:15454437).
In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.
Exposure time: 26 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710)
Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Cytoplasmic staining on rat cerebellum (PMID: 32899153).
The section was incubated with ab307710 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green).
Negative control: confocal image showing no staining on rat liver (PMID: 10729221).
The nuclear counterstain was DAPI (Blue). The section was incubated with ab307710 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat dorsal root ganglion (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green).
Panel A: merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on rat dorsal root ganglion.
Panel B: anti-Kv4.2 stained on the rat dorsal root ganglion.
Panel C: anti-NeuN stained in neurons of rat dorsal root ganglion.
Negative control: dorsal root ganglion (PMID: 19668710).
The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebellum (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green).
Panel A: merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on rat cerebellum.
Panel B: anti-Kv4.2 stained on the rat cerebellum.
Panel C: anti-NeuN stained in neurons of rat cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green).
Negative control: confocal image showing no staining on mouse liver (PMID: 10729221).
The nuclear counterstain was DAPI (Blue). The section was incubated with ab307710 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse dorsal root ganglion (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green).
Panel A: merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on mouse dorsal root ganglion.
Panel B: anti-Kv4.2 stained on the mouse dorsal root ganglion.
Panel C: anti-NeuN stained in neurons of mouse dorsal root ganglion.
Negative control: dorsal root ganglion (PMID: 19668710).The nuclear counterstain was DAPI (Blue).
The section was incubated in two rounds of staining: in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum (fresh) tissue labeling Kv4.2/KCND2 with ab307710 at 1/100 (5.32 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution (Green).
Panel A: merged staining of anti-Kv4.2 (ab307710, green) and anti-NeuN (ab190565, red) on mouse cerebellum.
Panel B: anti-Kv4.2 stained on the mouse cerebellum.
Panel C: anti-NeuN stained in neurons of mouse cerebellum. The nuclear counterstain was DAPI (Blue). The section was incubated in two rounds of staining: in the order of ab307710 and ab190565 for 1 hr at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 μg/mL dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Rat primary neuron cells cells labelling Kv4.2/KCND2 with ab307710 at 1/50 dilution (1μg)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells cells labelling Kv4.2/KCND2 with ab307710 at 1/50 dilution (1μg)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Kv4.2/KCND2 was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate 10 μg with ab307710 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307710 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat cerebellum tissue lysate 10 μg
Lane 2: ab307710 IP in Rat cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307710 in rat cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
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Kv4.2/KCND2 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 μg with ab307710 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307710 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10 μg
Lane 2: ab307710 IP in Mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab307710 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Negative control: no staining on rat liver.
The section was incubated with ab307710 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Negative control: no staining on mouse liver (PMID: 16293790).
The section was incubated with ab307710 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kv4.2/KCND2 antibody [EPR26384-89] (ab307710)
Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Kv4.2/KCND2 with ab307710 at 1/2000 (0.266 μg/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Cytoplasmic staining on mouse cerebellum (PMID: 17122039).
The section was incubated with ab307710 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab307710 has not yet been referenced specifically in any publications.