For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/l1cam-antibody-2c2-ab24345.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Cytoskeleton / ECM Cell Adhesion Cell Adhesion Molecules Liver
Share by email

Anti-L1CAM antibody [2C2] (ab24345)

  • Datasheet
Reviews (13)Q&A (6)References (40)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] (ab24345)
  • Western blot - Anti-L1CAM antibody [2C2] (ab24345)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] (ab24345)
  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
  • Western blot - Anti-L1CAM antibody [2C2] (ab24345)
  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)

Key features and details

  • Mouse monoclonal [2C2] to L1CAM
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

You may also be interested in

Assay
Product image
Coenzyme A Assay Kit (Fluorometric - Green) (ab138889)
Primary
Product image
Anti-L1CAM antibody [EPR18998] (ab182407)
Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

View more associated products

Overview

  • Product name

    Anti-L1CAM antibody [2C2]
    See all L1CAM primary antibodies
  • Description

    Mouse monoclonal [2C2] to L1CAM
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • WB: Mouse whole brain, Rat whole brain and Human whole brain tissue lysates. IHC-P: normal Human kidney and normal Rat kidney tissue sections. ICC: PC12 (undifferentiated and NGF-differentiated) and Neuro-2A (undifferentiated and TRA-differentiated). Primary hippocampal rat neurons/glia, DIV14 cells. Primary mouse neurons/glia, DIV14 cells.
  • General notes

    L1CAM can be detected between 200-220 kD. In brain samples it is typically seen at ~ 200 kD. When the protein is overexpressed in vitro it is often detected as a doublet with bands at 200 and 220 kD. The unglycosylated, unprocessed L1CAM is ~ 140-150 kDa. The protein has 21 putative N-glycosylation sites on the extracellular portion of the protein which, when they are all glycosylated, results in a detected MW of 200-220 kD depending upon how many residues are actually glycosylated. L1CAM can be cleaved by the metalloprotease ADAM10 resulting in fragments of 180 kD and 40 kD. L1CAM can also be cleaved by plasmin resulting in fragments of 140 kD and 80 kD. In theory, therefore, one could detect bands at ~220, 200, 180, 140, 80 and 40 kD.

    This product was changed from ascites to tissue culture supernatant on 08/Jul/2019. Lot numbers higher than GR3248431 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    2C2
  • Isotype

    IgG2b
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Liver
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-L1CAM antibody [2C2] - BSA and Azide free (ab264526)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Recombinant Protein

    • Recombinant Human L1CAM protein (His tag) (ab222980)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unsuitable for IHC-P in mouse tissue from in-house testing. 

ICC (1)
Use a concentration of 1 µg/ml.
WB (5)
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa.

Cleavage products observed at 60-80 kDa.

Notes
IHC-P
Use a concentration of 0.1 - 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unsuitable for IHC-P in mouse tissue from in-house testing. 

ICC
Use a concentration of 1 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 200 kDa.

Cleavage products observed at 60-80 kDa.

Target

  • Function

    Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.
  • Involvement in disease

    Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
    Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
    Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
    Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P32004 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3897 Human
    • Entrez Gene: 16728 Mouse
    • Entrez Gene: 50687 Rat
    • Omim: 308840 Human
    • SwissProt: P32004 Human
    • SwissProt: P11627 Mouse
    • SwissProt: Q05695 Rat
    • Unigene: 522818 Human
    • Unigene: 260568 Mouse
    • Unigene: 10378 Rat
    see all
  • Alternative names

    • Antigen identified by monoclonal antibody R1 antibody
    • CAML1 antibody
    • CD171 antibody
    • CD171 antigen antibody
    • HSAS antibody
    • HSAS1 antibody
    • Hyd antibody
    • L1 antibody
    • L1 cell adhesion molecule antibody
    • L1-NCAM antibody
    • L1cam antibody
    • L1CAM_HUMAN antibody
    • MASA antibody
    • MIC5 antibody
    • N CAML1 antibody
    • N-CAM-L1 antibody
    • NCAM-L1 antibody
    • NCAML1 antibody
    • Nerve-growth factor-inducible large external glycoprotein antibody
    • Neural cell adhesion molecule L1 antibody
    • NILE antibody
    • OTTHUMP00000025992 antibody
    • S10 antibody
    • SPG1 antibody
    see all

Images

  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
    Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)

    ab24345 staining L1CAM in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24345 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
    Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)

    ab24345 staining L1CAM in primary mouse neurons/glia, DIV14 (prepared from E18 mouse hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP) cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24345 at 1µg/ml and ab4674, Chicken polyclonal to GFAP. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150176, Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

    Also suitable in cells fixed with 4% paraformaldehyde (10 min).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
    Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)

    ab24345 staining L1CAM in undifferentiated Neuro-2A cells (top panel) and TRA-differentiated Neuro-2A cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab24345 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] (ab24345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] (ab24345)

    IHC image of L1CAM staining in a section of formalin-fixed paraffin-embedded normal rat kidney performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24345, 0.5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-L1CAM antibody [2C2] (ab24345)
    Western blot - Anti-L1CAM antibody [2C2] (ab24345)
    All lanes :

    Lane 1 : Mouse whole brain tissue lysate
    Lane 2 : Rat whole brain tissue lysate
    Lane 3 : Human whole brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Observed band size: 200 kDa why is the actual band size different from the predicted?
    Additional bands at: 60 kDa (possible cleavage fragment)



    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before ab24345 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] (ab24345)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [2C2] (ab24345)

    IHC image of L1CAM staining in a section of formalin-fixed paraffin-embedded normal human kidney* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab24345, 0.1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    This image was generated using the ascites version of the product.

  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
    Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)

    ab24345 staining L1CAM in undifferentiated PC12 cells (top panel) and NGF-differentiated PC12 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab24345 at 5μg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (shown in green) and ab150084, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolor red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This image was generated using the ascites version of the product.

  • Western blot - Anti-L1CAM antibody [2C2] (ab24345)
    Western blot - Anti-L1CAM antibody [2C2] (ab24345)This image is courtesy of Martin Grumet, Rutgers University, United States
    Anti-L1CAM antibody [2C2] (ab24345) at 1/1000 dilution + 30ug CNS protein

    Performed under reducing conditions.

    Observed band size: 200 kDa why is the actual band size different from the predicted?
    Additional bands at: 60-80 kDa (possible cleavage fragment)



    ab24345 recognizes one or two polypeptides of L1 or Ng-CAM corresponding to the full length protein (~200kDa) as well as 60-80 kDa  C-terminal cleavage products (as shown in the figure).

    This image was generated using the ascites version of the product.

  • Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)
    Immunocytochemistry - Anti-L1CAM antibody [2C2] (ab24345)Image from Donier E et al., PLoS One. 2012;7(7):e40674. Epub 2012 Jul 16. Fig 2.; doi:10.1371/journal.pone.0040674; July 16, 2012, PLoS ONE 7(7): e40674.

    Immunofluorescence analysis of COS7 cells transfected with full-length L1CAM (left) or truncated L1CAM (right), staining L1CAM (green) with ab24345.

    Cells were incubated with primary antibody (1/1000 in 1% goat serum + 0.3% Triton X-100 in PBS) and incubated overnight at 4°C. An AlexaFluor®488-conjugated anti-mouse IgG (1/700) was used as the secondary antibody. Nuclei were counterstained with bisbenzimide (blue).

    This image was generated using the ascites version of the product.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (40)

Publishing research using ab24345? Please let us know so that we can cite the reference in this datasheet.

ab24345 has been referenced in 40 publications.

  • Rudd JS  et al. Development of a reliable bovine neuronal cell culture system and labeled recombinant bovine herpesvirus type-1 for studying virus-host cell interactions. Virus Res 293:198255 (2021). PubMed: 33338533
  • Mishra PS & Raju TR A Simple and Efficient Method for Concomitant Isolation and Culture of Enriched Astroglial and Microglial Cells from the Rat Spinal Cord. Bio Protoc 10:e3501 (2020). PubMed: 33654728
  • Del Favero G  et al. Mycotoxin Altertoxin II Induces Lipid Peroxidation Connecting Mitochondrial Stress Response to NF-?B Inhibition in THP-1 Macrophages. Chem Res Toxicol 33:492-504 (2020). PubMed: 32022557
  • Yamamoto-Fukuda T  et al. L1CAM-ILK-YAP Mechanotransduction Drives Proliferative Activity of Epithelial Cells in Middle Ear Cholesteatoma. Am J Pathol N/A:N/A (2020). PubMed: 32360569
  • Gronska-Peski M  et al. L1cam curbs the differentiation of adult-born hippocampal neurons. Stem Cell Res 48:101999 (2020). PubMed: 32971459
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 19 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-L1CAM antibody [2C2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Melanoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 14 2021

Immunocytochemistry abreview for Anti-L1CAM antibody [2C2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 20°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 09 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-L1CAM antibody [2C2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 05 2021

Immunohistochemistry (Frozen sections) abreview for Anti-L1CAM antibody [2C2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin - epidermis)
Permeabilization
No
Specification
Skin - epidermis
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 16 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-L1CAM antibody [2C2]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH = 6, Citrate
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 05 2021

Western blot abreview for Anti-L1CAM antibody [2C2]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Melanoma)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Melanoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Praveen Agrawal

Verified customer

Submitted Mar 24 2017

Immunoprecipitation abreview for Anti-L1CAM antibody [2C2]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (Melanoma cell line MeWo)
Total protein in input
600 µg
Immuno-precipitation step
Protein G
Specification
Melanoma cell line MeWo
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Praveen Agrawal

Verified customer

Submitted Mar 24 2017

Western blot abreview for Anti-L1CAM antibody [2C2] - Neuronal Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Loading amount
30 µg
Specification
Brain - Cerebellar granule cells - DIV3
Treatment
100 ng/ml human BDNF for 6 minutes
Gel Running Conditions
Reduced Denaturing (8% SDS PAGE)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 12 2013

Question

I'm interested in your product ab24345.
However you don't have information in the website concerning the antibody concentration. You just provide information about the volume (size). Could you please give me additional information?
Thanks in advance,

Read More

Abcam community

Verified customer

Asked on Mar 13 2013

Answer

Thank you for your enquiry.

I can confirm that ab24345 antibody is sold as ascites. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for ascites, concentration of antibody is known to very between 5 - 10 mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Mar 13 2013

Question

We require anti-L1 CAM antibody that detects full length as well as all its cleaved products in mouse brain. I have come across one of your product anti-L1CAM antibody [2C2] - Neuronal Marker (ab24345) that shows 200 kD, 80 kD and 60 kD bands. In addition, it is mentioned that it may detect other cleaved products theoretically. However, before ordering the antibody we want to be sure that it also detects all the bands (full length of 220, 200kD as well as 180, 140, 80 and 40 kD).

Is there is any possibility to provide a very small aliqout of the same (may be 2-5 microlitre) to check whether it serves our interest. Alternatively, if you are sure for what is mentioned in the catalogue, we may order it on approval basis.

Read More

Abcam community

Verified customer

Asked on Dec 27 2012

Answer

Thank you for your interest in this antibody. Two customers sent their mouse western blot results, both showing detection of bands that they assumed represented the full-length protein and a fragment. However, we do not guarantee that the antibody will detect all possible fragments, and we do not offer free samples. Here are links to the two reviews.

https://www.abcam.com/L1CAM-antibody-2C2-Neuronal-Marker-ab24345-reviews.html?intabreviewid=8617

https://www.abcam.com/L1CAM-antibody-2C2-Neuronal-Marker-ab24345-reviews.html?intabreviewid=8066

Given these results, it seems unlikely that the antibody will be sensitive enough to detect all fragments, assuming the fragments (220, 200, 180, 140, 80 and 40 kDa) are present in these samples.

Read More

Abcam Scientific Support

Answered on Dec 27 2012

1-10 of 19 Abreviews or Q&A

  •  Previous
  • 1
  • 2
  • Next 

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.