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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
  • Flow Cytometry - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
  • Immunoprecipitation - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
  • Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23241-224] to L1CAM - BSA and Azide free
  • Suitable for: Flow Cyt, IP, ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Unconjugated

Overview

  • Product name

    Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free
    See all L1CAM primary antibodies

  • Description

    Rabbit monoclonal [EPR23241-224] to L1CAM - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, ICC/IF, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human brain tissue lysate; A375 and MCF7 whole cell lysates. IHC-P: Human kidney, spleen and cerebellum tissue. Flow Cyt: A375 cells. IP: A375 whole cell lysate.

  • General notes

    ab272321 is the carrier-free version of ab270455.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 250, 80 kDa (predicted molecular weight: 140 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 250, 80 kDa (predicted molecular weight: 140 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons.

  • Involvement in disease

    Defects in L1CAM are the cause of hydrocephalus due to stenosis of the aqueduct of Sylvius (HSAS) [MIM:307000]. Hydrocephalus is a condition in which abnormal accumulation of cerebrospinal fluid in the brain causes increased intracranial pressure inside the skull. This is usually due to blockage of cerebrospinal fluid outflow in the brain ventricles or in the subarachnoid space at the base of the brain. In children is typically characterized by enlargement of the head, prominence of the forehead, brain atrophy, mental deterioration, and convulsions. In adults the syndrome includes incontinence, imbalance, and dementia. HSAS is characterized by mental retardation and enlarged brain ventricles.
    Defects in L1CAM are the cause of mental retardation-aphasia-shuffling gait-adducted thumbs syndrome (MASA) [MIM:303350]; also known as corpus callosum hypoplasia, psychomotor retardation, adducted thumbs, spastic paraparesis, and hydrocephalus or CRASH syndrome. MASA is an X-linked recessive syndrome with a highly variable clinical spectrum. Main clinical features include spasticity and hyperreflexia of lower limbs, shuffling gait, mental retardation, aphasia and adducted thumbs. The features of spasticity have been referred to as complicated spastic paraplegia type 1 (SPG1). Some patients manifest corpus callosum hypoplasia and hydrocephalus. Inter- and intrafamilial variability is very wide, such that patients with hydrocephalus, MASA, SPG1, and agenesis of corpus callosum can be present within the same family.
    Defects in L1CAM are the cause of spastic paraplegia X-linked type 1 (SPG1) [MIM:303350]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
    Note=Defects in L1CAM may contribute to Hirschsprung disease by modifying the effects of Hirschsprung disease-associated genes to cause intestinal aganglionosis.
    Defects in L1CAM are a cause of partial agenesis of the corpus callosum (ACCPX) [MIM:304100]. A syndrome characterized by partial corpus callosum agenesis, hypoplasia of inferior vermis and cerebellum, mental retardation, seizures and spasticity. Other features include microcephaly, unusual facies, and Hirschsprung disease in some patients.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.
    Contains 5 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen identified by monoclonal antibody R1 antibody
      • CAML1 antibody
      • CD171 antibody
      • CD171 antigen antibody
      • HSAS antibody
      • HSAS1 antibody
      • Hyd antibody
      • L1 antibody
      • L1 cell adhesion molecule antibody
      • L1-NCAM antibody
      • L1cam antibody
      • L1CAM_HUMAN antibody
      • MASA antibody
      • MIC5 antibody
      • N CAML1 antibody
      • N-CAM-L1 antibody
      • NCAM-L1 antibody
      • NCAML1 antibody
      • Nerve-growth factor-inducible large external glycoprotein antibody
      • Neural cell adhesion molecule L1 antibody
      • NILE antibody
      • OTTHUMP00000025992 antibody
      • S10 antibody
      • SPG1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

      Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling L1CAM with ab270455 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the molecular layer of the human cerebellum (PMID: 12514225). The section was incubated with ab270455 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

    • Flow Cytometry - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
      Flow Cytometry - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

      Flow cytometric analysis of A549 (Human lung carcinoma epithelial cell, Left) / A375 (Human malignant melanoma epithelial cell, Right) cells labelling L1CAM with ab270455 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      A549 is negative or very low expression cell line for L1CAM (PMID: 23511563).

      Gated on viable cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

    • Immunoprecipitation - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
      Immunoprecipitation - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

      L1CAM was immunoprecipitated from 0.35 mg A375 (human malignant melanoma epithelial cell), whole cell lysate using ab270455 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab270455 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: A375 (human malignant melanoma epithelial cell), whole cell lysate 10ug

      Lane 2: ab270455 IP in A375 whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab270455 in A375 whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5.5 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling L1CAM with ab270455 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the nerve in human spleen (PMID: 26743472). The section was incubated with ab270455 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling L1CAM with ab270455 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on distal renal tubules in human kidney.The section was incubated with ab270455 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab270455).

    • Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)
      Anti-L1CAM antibody [EPR23241-224] - BSA and Azide free (ab272321)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab272321? Please let us know so that we can cite the reference in this datasheet.

    ab272321 has been referenced in 1 publication.

    • Norman M  et al. L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma. Nat Methods 18:631-634 (2021). PubMed: 34092791

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