Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437)
![Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437)](https://www.abcam.com/ps/products/174/ab174437/Images/ab174437-199863-anti-l2-hnk-1-carbohydrate-antibody-zn-12-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437)")
Key features and details
- Mouse monoclonal [ZN-12] to L2/HNK-1 Carbohydrate
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] -
Description
Mouse monoclonal [ZN-12] to L2/HNK-1 Carbohydrate -
Host species
Mouse -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Zebrafish
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- SH-SY5Y cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.9% HEPES buffered saline -
Concentration information loading... -
Purity
SDS-PAGE -
Purification notes
ab174437 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
ZN-12 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174437 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
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ICC/IF
Use a concentration of 5 µg/ml. |
Images
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Immunocytochemistry/ Immunofluorescence - Anti-L2/HNK-1 Carbohydrate antibody [ZN-12] (ab174437)
Immunocytochemistry with SH-SY5Y cells stained with ab174437 in red and DAPI in blue, as a counter stain. Fixation: Paraformaldehyde fixed (4%, 20 min) Permeabilization: Triton X-100 permeabilized (0.1%, 10 min) Blocking: 10% Goat Serum/PBS (1 hour) Primary: ab174437 5 µg/mL (10% Goat Serum/PBS, 2hr at room temperature or overnight at 4°C). Secondary: Alexa 594 goat anti-mouse IgG1 used at a 1/1000 dilution in 10% Goat Serum/PBS for 1hr at RT. Washing: 3x 1% Goat Serum/PBS, 10 minutes/wash DAPI: 2 µM in 1% Goat Serum/PBS, 10 minutes.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab174437 has not yet been referenced specifically in any publications.
