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RecombinantRabMAb

Recombinant Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)

Reviews (3)Q&A (5)References (70)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)
  • Western blot - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)
  • Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1564] to Lactate Dehydrogenase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Lactate Dehydrogenase antibody [EPR1564]
    See all Lactate Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR1564] to Lactate Dehydrogenase

  • Host species

    Rabbit

  • Specificity

    The immunogen for ab101562 has 93% homology with L-lactate dehydrogenase C chain (P07864).

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, MCF-7, A375 and 293T cell lysates, Human Muscle tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P (1)
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is strongly recommended.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is strongly recommended.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Pathway

    Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

  • Involvement in disease

    Defects in LDHA are the cause of glycogen storage disease type 11 (GSD11) [MIM:612933]. A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

  • Sequence similarities

    Belongs to the LDH/MDH superfamily. LDH family.

  • Post-translational
    modifications

    ISGylated.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cell proliferation-inducing gene 19 protein antibody
    • GSD11 antibody
    • L lactate dehydrogenase B chain antibody
    • L-lactate dehydrogenase A chain antibody
    • Lactate dehydrogenase A antibody
    • Lactate dehydrogenase B antibody
    • Lactate dehydrogenase c variant 1 antibody
    • Lactate dehydrogenase c variant 3 antibody
    • Lactate dehydrogenase c variant 4 antibody
    • Lactate dehydrogenase C4 antibody
    • Lactate dehydrogenase H chain antibody
    • Lactate dehydrogenase M antibody
    • LDH A antibody
    • LDH B antibody
    • LDH H antibody
    • LDH heart subunit antibody
    • LDH M antibody
    • LDH muscle subunit antibody
    • LDH-A antibody
    • LDH-M antibody
    • LDH1 antibody
    • ldha antibody
    • LDHA_HUMAN antibody
    • LDHBD antibody
    • LDHM antibody
    • MS1111 antibody
    • PIG19 antibody
    • Proliferation inducing gene 19 antibody
    • Proliferation-inducing gene 19 antibody
    • Renal carcinoma antigen NY REN 46 antibody
    • Renal carcinoma antigen NY-REN-59 antibody
    • TRG 5 antibody
    • TRG5 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)

    Staining of Human Lactate Dehydrogenase in a Formalin/PFA-fixed paraffin-embedded section of human Muscle using ab101526 at a dilution of 1/500.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)
    Western blot - Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)
    All lanes : Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : A375 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 37 kDa

  • Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)
    Anti-Lactate Dehydrogenase antibody [EPR1564] (ab101562)

References (70)

Publishing research using ab101562? Please let us know so that we can cite the reference in this datasheet.

ab101562 has been referenced in 70 publications.

  • Xu W  et al. m6A RNA methylation-mediated NDUFA4 promotes cell proliferation and metabolism in gastric cancer. Cell Death Dis 13:715 (2022). WB ; Human . PubMed: 35977935
  • Tang HY  et al. PDGFRβ modulates aerobic glycolysis in osteosarcoma HOS cells via the PI3K/AKT/mTOR/c-Myc pathway. Biochem Cell Biol 100:75-84 (2022). PubMed: 34678088
  • Zhou X  et al. BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) promotes lung adenocarcinoma by interacting with Zinc Finger Protein ZNF143 and regulating glycolysis. Bioengineered 13:2471-2485 (2022). PubMed: 35068350
  • Yang W  et al. CRNDE silencing promotes apoptosis and enhances cisplatin sensitivity of colorectal carcinoma cells by inhibiting the Akt/mTORC1-mediated Warburg effect. Oncol Lett 23:70 (2022). PubMed: 35069879
  • Guo JQ  et al. PDGF-BB/PDGFRβ promotes epithelial-mesenchymal transition by affecting PI3K/AKT/mTOR-driven aerobic glycolysis in Wilms' tumor G401 cells. Cell Biol Int 46:907-921 (2022). PubMed: 35165984
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Lactate Dehydrogenase antibody [EPR1564]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6.00, overnight at 60*C
Permeabilization
No
Specification
Skin
Blocking step
UVblock as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 17 2022

Western blot abreview for Anti-LDHA antibody [EPR1564]

 Poor
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain lysate (lys) and myelin (mye))
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
15 µg
Specification
brain lysate (lys) and myelin (mye)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 22 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-LDHA antibody [EPR1564]

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary Oligodendrocytes)
Specification
primary Oligodendrocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 21 2012

Question

ich habe von Ihnen das Angebot über einen 100%igen Abreview-Rabatt bekommen, wenn ich den LDHA Antikörper ab101562 teste. Ich habe diesen Antikörper im Western Blot und immunhistologisch auf Paraffin- und Vibratomschnitten sowie immuncytologisch auf Ratten-Zellen. Schicke ich Ihnen einfach die Ergebnisse? Oder submitte ich meine Ergebnisse auf Ihrer Homepage und muss mich zunächst registrieren? Bekomme ich dann im Anschluss automatisch den Rabattcode zugesendet?

Mit freundlichen Grüße,

Read More
Answer

Vielen Dank für Ihre Email.


Ich kann Ihnen bestätigen, dass Sie einfach einen Abreview über die Homepage einsenden können. Wenn sie auf das datenblatt von ab101562 gehen gibt es direkt unter dem Produktmanen einen Link "Submit an Abreview".

Oder Sie wählen den Reiter "Abreview" und benutzen den Link dort.

Sie müssen sich anmelden um einen Abreview zu schrieben. Sie bekommen auch Abpoints, die dann auf Ihr Konto gutgeschrieben werden.

Wenn Sie den Abreview ausfüllen geben Sie bitte den Gutschien Code
in den "additional notes" an.

Wir freuen uns auf den Abreview. Bitte lassen Sie mich wissen, falls Sie weitere Fragen haben.

Read More

Question

I don't fully understand the difference of your anti-LDHA(101018) and anti-LDH isoenzyme V(85472)antibody. As I know that LDH isoenzyme II-V contain M protein subunits encoded by the LDHA gene, so those two antibodys should detect all LDHs excxept LDH-1. Please let me know your answer. Thank you very much.

Read More
Answer

Thank you for your reply and for your patience.


I have talked to the lab and we cannot guarantee that ab101018, ab85472 and ab101562 24will not cross react with other LDH family members as the sequence homology between the family members is too high to say that with absolute certainty that they will not cross react.


Please let me know if you have any additional questions.

Read More

Question

- searching reactives for IHC-P of LDH 5 - including revelation kit, blocking reagents and antigen retrieval solution

Read More
Answer

Merci pour votre appel de hier. Je suis désolée du retard de ma réponse.

Voici donc la liste des produits que nous avions discuté ainsi que les liens pour les protocoles que nous avons en ligne.

Les anticorps qui ont été testé contre la protéine humaine ainsi que sur coupes paraffine:
contre la LDH total, c'est le ab52488 et contre la LDH isoenzyme V c'est le ab101562.
https://www.abcam.com/Lactate-Dehydrogenase-antibody-EP1566Y-ab52488.html (or use the following: https://www.abcam.com/Lactate-Dehydrogenase-antibody-EP1566Y-ab52488.html).
https://www.abcam.com/LDHA-antibody-EPR1564-ab101562.html (or use the following: https://www.abcam.com/LDHA-antibody-EPR1564-ab101562.html).

Le kit de révélation pour détecter l'anticorps primaire est le kit ab80436. Comme vous utilisez ce kit, vous n'avez pas besoin de bloquer la biotine endogène ni d'un autre agent bloquant les protéines, car il contient déjà la solution de blocage ("protein block").
https://www.abcam.com/EXPOSE-Mouse-and-Rabbit-Specific-HRPDAB-Detection-IHC-Kit-ab80436.html (or use the following: https://www.abcam.com/EXPOSE-Mouse-and-Rabbit-Specific-HRPDAB-Detection-IHC-Kit-ab80436.html).

Les solutions de démasquages sont le PH 9 ab93684 pour un démasquage à pH 9 et le ab93678 pour un démasquage à pH 6.
https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-90-ab93684.html (or use the following: https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Tris-EDTA-Buffer-pH-90-ab93684.html).
https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Citrate-Buffer-pH-60-ab93678.html (or use the following: https://www.abcam.com/Antigen-Retrieval-Buffer-100X-Citrate-Buffer-pH-60-ab93678.html).

La solution de blocage est le ab64218 pour bloquer l'activité endogène de peroxydase. Cet étape de blocage est très important si vous travaillez avec des tissues qui contiennent de l'activité endogène de peroxydase, comme par exemple le poumon. Veuillez ne pas hésiter à nous rappeler pour discuter ceci en plus de détails.
https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab64218.html (or use the following: https://www.abcam.com/Hydrogen-Peroxide-Blocking-Reagent-ab64218.html).

Voici les liens pour les protocoles d'immunohistochimie. Ils contiennent beaucoup des informations utiles.

https://www.abcam.com/index.html?pageconfig=resource&rid=11757
le même protocole en pdf: https://www.abcam.com/ps/pdf/protocols/ihc_p.pdf
https://www.abcam.com/index.html?pageconfig=resource&rid=11384
https://www.abcam.com/index.html?pageconfig=popular_protocols

Voici aussi ici des liens plus spécifiques, comme pour le démasquage et pour les kits de détection.
https://www.abcam.com/index.html?pageconfig=searchresults&pt=6&search=antigen retrieval
https://www.abcam.com/index.html?pageconfig=resource&rid=12865&source=pagetrap&viapagetrap=expose

J'espère que ces informations vous sont utiles. N'hésitez pas à nous rappeler pour plus d'information.

Read More

Question

danke für Ihr Angebot. Gerne möchte ich es nutzen und den Antikörper 101562
testen. Bitte schicken Sie mir den Rabattcode zu. Sollte ich diesen bei der
Bestellung von 101562 bereits angeben oder ist das nur für die Bestellung
anderer Produkte anzugeben?
Mit freundlichen Grüßen,

Read More
Answer

DISCOUNT CODE: 00000

Ablaufdatum: 06. Oktober 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab101562 in Maus zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer 100%ABR-E71AB, deckt den Wert von ab101562 ab und kann zum Kauf weiterer Produkte eingesetzt werden. Bitte geben Sie den Code erst bei der Bestellung andere Produktean.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Maus eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der Bestellung nachEinreichen des Abreviewunsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

Read More

Question

sELISA with human serum samples
will test ab85472, ab101562, ab77945 in sELISA

Read More
Answer

Thank you for your phone call.
As we discussed I am sending you the 3 discount codes for the 2 antibodies as well as the one protein, all of which have not yet been tested in sandwich ELISA.
DISCOUNT CODE: xxx for ab85472
DISCOUNT CODE: xxx for ab101562
DISCOUNT CODE: xxx for ab77945
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab85472, ab101562, ab77945 in sandwich ELISA. Each code will give you either 1 free PRIMARY ANTIBODY or 1 FREE PROTEIN before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include the codes in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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For licensing inquiries, please contact partnerships@abcam.com