Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482)
Key features and details
- Goat polyclonal to Lactate Dehydrogenase B/LDH-B
- Suitable for: WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-Lactate Dehydrogenase B/LDH-B antibody
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
Description
Goat polyclonal to Lactate Dehydrogenase B/LDH-B -
Host species
Goat -
Specificity
Reported variants represent identical protein: NP_002291.1, NP_001167568.1. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Predicted to work with: Rabbit, Cow, Dog, Chimpanzee, Cynomolgus monkey
-
Immunogen
Synthetic peptide corresponding to Human Lactate Dehydrogenase B/LDH-B aa 250 to the C-terminus (internal sequence) (Cysteine residue). (Near C-terminus) (NP_002291.1)
Database link: P07195 -
Positive control
- WB: PIg, human, rat and mouse heart lysates.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 37 kDa). 1 hour primary incubation is recommended for this product. |
Target
-
Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 465337 Chimpanzee
- Entrez Gene: 281275 Cow
- Entrez Gene: 101925612 Cynomolgus monkey
- Entrez Gene: 3945 Human
- Entrez Gene: 16832 Mouse
- Entrez Gene: 100009106 Rabbit
- Entrez Gene: 24534 Rat
- Omim: 150100 Human
see all -
Alternative names
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
-
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482)Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/ml + Pig heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 37 kDaPrimary incubation for 1 hour.
-
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482)All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody (ab240482) at 0.1 µg/ml
Lane 1 : Human heart lysate in RIPA buffer
Lane 2 : Mouse heart lysate in RIPA buffer
Lane 3 : Rat heart lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDaPrimary incubation for 1 hour.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (1)
ab240482 has been referenced in 1 publication.
- Hao LS et al. Correlation and expression analysis of hypoxia-inducible factor 1a, glucose transporter 1 and lactate dehydrogenase 5 in human gastric cancer. Oncol Lett 18:1431-1441 (2019). PubMed: 31423208
