Recombinant Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)
![Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)](https://www.abcam.com/ps/products/53/ab53292/Images/ab53292-448904-recombinant-anti-lactate-dehydrogenase-bldh-b-antibody-ep1565y-western-blot-hek-293t-lysates.jpg "Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y]
See all Lactate Dehydrogenase B/LDH-B primary antibodies -
Description
Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase B/LDH-B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra) or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Lactate Dehydrogenase B/LDH-B aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P07195 -
Positive control
- WB: Hap1, HEK-293T and HeLa cell lysates. IHC-P: Human heart muscle.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1565Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/50000 - 1/200000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
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| IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/50000 - 1/200000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Pathway
Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1. -
Involvement in disease
Note=Defects in LDHB result in deficiency of lactate dehydrogenase, a condition with no clear symptomatic consequences. Lactate dehydrogenase deficiency can probably be considered a non-disease. -
Sequence similarities
Belongs to the LDH/MDH superfamily. LDH family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3945 Human
- Omim: 150100 Human
- SwissProt: P07195 Human
- Unigene: 446149 Human
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Alternative names
- Epididymis secretory protein Li 281 antibody
- HEL S 281 antibody
- L lactate dehydrogenase B chain antibody
see all
Images
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Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) at 1/5000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : LDHB knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDaLanes 1- 2: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - Anti-Vinculin antibody [VIN-54] observed at 124 kDa.
ab53292 was shown to react with Lactate Dehydrogenase B/LDH-B in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255403 (knockout cell lysate ab263761) was used. Wild-type HEK-293T and LDHB knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab53292 and Anti-Vinculin antibody [VIN-54] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)](https://www.abcam.com/ps/products/53/ab53292/Images/ab53292-327967-anti-lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-western-blot.jpg)
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) at 1/50000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : LDHB (Lactate Dehydrogenase B) knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 37 kDaLanes 1 - 3: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab53292 was shown to specifically react with Lactate Dehydrogenase B in wild-type HAP1 cells as signal was lost in LDHB (Lactate Dehydrogenase B) knockout cells. Wild-type and LDHB (Lactate Dehydrogenase B) knockout samples were subjected to SDS-PAGE. Ab53292 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)](https://www.abcam.com/ps/products/53/ab53292/Images/ab53292-66085-anti-lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-immunohistochemistry.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)Human heart muscle labeled with ab53292 at 1/100 - 1/250 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)](https://www.abcam.com/ps/products/53/ab53292/Images/ab53292-66086-anti-lactate-dehydrogenase-b-ldh-b-antibody-ep1565y-western-blot.png)
Western blot - Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) + HeLa cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP
Predicted band size: 37 kDa
Observed band size: 37 kDa -
![Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)](https://www.abcam.com/ps/products/53/ab53292/Images/ab53292-7-benefits-of-recombinant-antibodies.png)
Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab53292 has been referenced in 14 publications.
- Wang R et al. Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity. Front Oncol 12:821620 (2022). PubMed: 35669414
- Chen Y et al. Development of a rational strategy for integration of lactate dehydrogenase A suppression into therapeutic algorithms for head and neck cancer. Br J Cancer 124:1670-1679 (2021). PubMed: 33742144
- Du J et al. RIPK1 dephosphorylation and kinase activation by PPP1R3G/PP1γ promote apoptosis and necroptosis. Nat Commun 12:7067 (2021). PubMed: 34862394
- Tasdogan A et al. Metabolic heterogeneity confers differences in melanoma metastatic potential. Nature 577:115-120 (2020). PubMed: 31853067
- Johnston AN et al. Necroptosis-blocking compound NBC1 targets heat shock protein 70 to inhibit MLKL polymerization and necroptosis. Proc Natl Acad Sci U S A 117:6521-6530 (2020). PubMed: 32156734