Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] (ab112996)
![Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/ab112996-125716-anti-lactate-dehydrogenase-b-ldh-b-antibody-10e6aa9-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)")
Key features and details
- Mouse monoclonal [10E6AA9] to LDHA + LDHB
- Suitable for: ICC/IF, IP, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Lactate Dehydrogenase LDHA + LDHB antibody [10E6AA9] -
Description
Mouse monoclonal [10E6AA9] to LDHA + LDHB -
Host species
Mouse -
Specificity
Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques. -
Tested applications
Suitable for: ICC/IF, IP, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa cells and cell lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab112996 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
Clonality
Monoclonal -
Clone number
10E6AA9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
|
IP
Use at an assay dependent concentration. |
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Flow Cyt
Use a concentration of 1 - 2 µg/ml. Triton permeabilization required. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)
ab112996 at 2ug/ml staining Lactate Dehydrogenase B/LDH-B in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). The target protein locates mainly to the cytoplasm.
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![Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/ab112996-125715-anti-lactate-dehydrogenase-b-ldh-b-antibody-10e6aa9-flow-cytometry.jpg)
Flow Cytometry - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)ab112996 at 1ug/ml staining Lactate Dehydrogenase B/LDH-B in Triton X-100 permeabilized HeLa cells by flow cytometry (blue). Isotype control antibody (red).
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![Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)](https://www.abcam.com/ps/products/112/ab112996/Images/ab112996-125714-anti-lactate-dehydrogenase-b-ldh-b-antibody-10e6aa9-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Lactate Dehydrogenase B/LDH-B antibody [10E6AA9] (ab112996)The 36.6 kDa LDHB protein along with 36.7kDa LDHA protein were immunoprecipitated from Hela cell lysate Hela cell lysate with ab112996. The identities of the proteins were confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab112996 has been referenced in 1 publication.
- Ran F et al. miR-1224-3p Promotes Breast Cancer Cell Proliferation and Migration through PGM5-Mediated Aerobic Glycolysis. J Oncol 2021:5529770 (2021). PubMed: 33986801
