Recombinant Anti-LAG-3 antibody [BLR027F] (ab243884)
![Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884)](https://www.abcam.com/ps/products/243/ab243884/Images/ab243884-334327-anti-lymphocyte-activation-gene-3-antibody-blr027f-western-blot.jpg "Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR027F] to LAG-3
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-LAG-3 antibody [BLR027F]
See all LAG-3 primary antibodies -
Description
Rabbit monoclonal [BLR027F] to LAG-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human LAG-3 aa 500-525 (C terminal). The exact sequence is proprietary. NP_002277.4 and Gene ID 3902.
Database link: P18627 -
Positive control
- WB: HDLM-2 whole cell lysate. IP: HDLM-2 whole cell lysate. ICC: HDLM-2 cells. IHC-P: Human tonsil tissue.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR027F -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
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| ICC |
1/100 - 1/500.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
Use 12µl/0.5mg lysate. |
| Notes |
|---|
|
WB
1/1000. |
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ICC
1/100 - 1/500. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. Use 12µl/0.5mg lysate. |
Target
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Function
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
Tissue specificity
On cell surface of activated NK and T-lymphocytes. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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Alternative names
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
Images
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Western blot - Anti-LAG-3 antibody [BLR027F] (ab243884)
Western blot analysis using ab243884 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (50 µg).
Lane 2: RPMI 8226 whole cell lysate (50 µg).
Lane3: Jurkat whole cell lysate (50 µg).
Lane 4: MJ whole cell lysate (50 µg).
Lane 5: HEK-293T whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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![Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (ab243884)](https://www.abcam.com/ps/products/243/ab243884/Images/ab243884-334328-anti-lymphocyte-activation-gene-3-antibody-blr027f-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LAG-3 antibody [BLR027F] (ab243884)LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243884 at 12 µl per reaction. Western blot was performed on the immunoprecipitate using ab243884 at 1/1000 dilution.
Lane 1: ab243884 IP in HDLM-2 whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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![Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] (ab243884)](https://www.abcam.com/ps/products/243/ab243884/Images/ab243884-334326-anti-lymphocyte-activation-gene-3-antibody-blr027f-immunocytochemistry.jpg)
Immunocytochemistry - Anti-LAG-3 antibody [BLR027F] (ab243884)Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243884 at 1/100 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)](https://www.abcam.com/ps/products/243/ab243884/Images/ab243884-334325-anti-lymphocyte-activation-gene-3-antibody-blr027f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243884 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)](https://www.abcam.com/ps/products/243/ab243884/Images/ab243884-467815-anti-lag-3-antibody-blr027f-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR027F] (ab243884)Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 (red) using ab243884 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).
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![Anti-LAG-3 antibody [BLR027F] (ab243884)](https://www.abcam.com/ps/products/243/ab243884/Images/ab243884-5-benefits-of-recombinant-antibodies.png)
Anti-LAG-3 antibody [BLR027F] (ab243884)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243884 has not yet been referenced specifically in any publications.