Recombinant Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)
![Western blot - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)](https://www.abcam.com/ps/products/243/ab243885/Images/ab243885-3-ab243885WB.jpg "Western blot - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR028F] to LAG-3 - BSA free
- Suitable for: WB, ICC, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-LAG-3 antibody [BLR028F] - BSA free
See all LAG-3 primary antibodies -
Description
Rabbit monoclonal [BLR028F] to LAG-3 - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human LAG-3 (extracellular).
Database link: P18627 -
Positive control
- WB: HDLM-2 whole cell lysate. IP: HDLM-2 whole cell lysate. ICC: HDLM-2 cells. IHC-P: Human tonsil tissue.
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General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR028F -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000.
|
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| ICC |
1/100 - 1/500.
|
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| IP |
Use at an assay dependent concentration.
Use 6µl/0.5mg lysate. |
| Notes |
|---|
|
WB
1/1000. |
|
ICC
1/100 - 1/500. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
IP
Use at an assay dependent concentration. Use 6µl/0.5mg lysate. |
Target
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Function
Involved in lymphocyte activation. Binds to HLA class-II antigens. -
Tissue specificity
On cell surface of activated NK and T-lymphocytes. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3902 Human
- Omim: 153337 Human
- SwissProt: P18627 Human
- Unigene: 409523 Human
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Alternative names
- CD223 antibody
- CD223 antigen antibody
- FDC protein antibody
see all
Images
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Western blot - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)
Western blot analysis using ab243885 at 1/1000 dilution.
Lane 1: HDLM-2 whole cell lysate (5 µg).
Lane 2: RPMI 8226 whole cell lysate (5 µg).
Lane3: Jurkat whole cell lysate (5 µg).
Lane 4: MJ whole cell lysate (5 µg).
Lane 5: HEK-293T whole cell lysate (5 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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![Immunoprecipitation - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)](https://www.abcam.com/ps/products/243/ab243885/Images/ab243885-334332-anti-lymphocyte-activation-gene-3-antibody-blr028f-immunoprecipitation.jpg)
Immunoprecipitation - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)LAG-3 was immunoprecipitated from 0.5 mg HDLM-2 whole cell lysate with ab243885 at 6 µl per reaction. Western blot was performed on the immunoprecipitate using ab243885 at 1/1000 dilution.
Lane 1: ab243885 IP in HDLM-2 whole cell lysate.
Lane 2: Control IgG IP in HDLM-2 whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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![Immunocytochemistry - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)](https://www.abcam.com/ps/products/243/ab243885/Images/ab243885-334330-anti-lymphocyte-activation-gene-3-antibody-blr028f-immunocytochemistry.jpg)
Immunocytochemistry - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)Formalin-fixed, paraffin-embedded HDLM-2 cells labeling LAG-3 using ab243885 at 1/200 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)](https://www.abcam.com/ps/products/243/ab243885/Images/ab243885-334329-anti-lymphocyte-activation-gene-3-antibody-blr028f-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 using ab243885 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)](https://www.abcam.com/ps/products/243/ab243885/Images/ab243885-467820-anti-lag-3-antibody-blr028f-bsa-free-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)Formalin-fixed, paraffin-embedded human tonsil tissue stained for LAG-3 (red) using ab243885 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DABI staining.
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![Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)](https://www.abcam.com/ps/products/243/ab243885/Images/ab243885-5-benefits-of-recombinant-antibodies.png)
Anti-LAG-3 antibody [BLR028F] - BSA free (ab243885)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab243885 has not yet been referenced specifically in any publications.