Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)](https://www.abcam.com/ps/datasheet/images/1/ab1944/Lambda-Free-Light-Chain-Primary-antibodies-ab1944-1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)")
Key features and details
- Mouse monoclonal [3D12] to Lambda Free Light Chain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Lambda Free Light Chain antibody [3D12] -
Description
Mouse monoclonal [3D12] to Lambda Free Light Chain -
Host species
Mouse -
Specificity
Specific for independent constantly expressed epitope of lambda chain. Reacts with monomer and dimer forms of free lambda chains but not with lambda chain-bearing immunoglobulins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Human Lambda Free Light Chain.
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Positive control
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal tonsil.
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General notes
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purity tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
3D12 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
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| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Relevance
standard
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Free Light Chain antibody [3D12] (ab1944)
IHC image of Lambda Free Light Chain staining in Human normal tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with abX1944, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab1944 has not yet been referenced specifically in any publications.