Recombinant Anti-Lambda Light chain antibody [1-155-2] (ab235129)
![Flow Cytometry - Anti-Lambda Light chain antibody [1-155-2] (ab235129)](https://www.abcam.com/ps/products/235/ab235129/Images/ab235129-368867-AntiLambda-Light-chain-11552-BSA-Azide-free-flow-cytometry-human-peripheral-blood-mononuclear-cells.jpg "Flow Cytometry - Anti-Lambda Light chain antibody [1-155-2] (ab235129)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1-155-2] to Lambda Light chain
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lambda Light chain antibody [1-155-2]
See all Lambda Light chain primary antibodies -
Description
Mouse monoclonal [1-155-2] to Lambda Light chain -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human peripheral blood mononuclear cells (PBMC).
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1-155-2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235129 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1.105 µg/ml.
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| Notes |
|---|
|
Flow Cyt
Use a concentration of 1.105 µg/ml. |
Target
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Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
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Flow Cytometry - Anti-Lambda Light chain antibody [1-155-2] (ab235129)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling Lambda Light chain with ab235129 at 1.105µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or ab235129 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Anti-Lambda Light chain antibody [1-155-2] (ab235129)](https://www.abcam.com/ps/products/235/ab235129/Images/ab235129-2-benefits-of-recombinant-antibodies.png)
Anti-Lambda Light chain antibody [1-155-2] (ab235129)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235129 has not yet been referenced specifically in any publications.