Recombinant Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free (ab270744)
![Flow Cytometry - Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free (ab270744)](https://www.abcam.com/ps/products/270/ab270744/Images/ab270744-389598-AntiLambdaLightchain11552BSAAzidefreeflowcytometryhumanperipheralbloodmononuclearcells.jpg "Flow Cytometry - Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free (ab270744)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [1-155-2] to Lambda Light chain - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free
See all Lambda Light chain primary antibodies -
Description
Mouse monoclonal [1-155-2] to Lambda Light chain - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Human peripheral blood mononuclear cells (PBMC).
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General notes
ab270744 is the carrier-free version of ab235129.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1-155-2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab270744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use a concentration of 1.105 µg/ml.
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| Notes |
|---|
|
Flow Cyt
Use a concentration of 1.105 µg/ml. |
Target
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Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
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Flow Cytometry - Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free (ab270744)
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling Lambda Light chain with ab235129 at 1.105µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or ab235129 (Right). Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulkation containing PBS, glycerol, BSA and sodium azide (ab235129).
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![Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free (ab270744)](https://www.abcam.com/ps/products/270/ab270744/Images/ab270744-2-benefits-of-recombinant-antibodies.png)
Anti-Lambda Light chain antibody [1-155-2] - BSA and Azide free (ab270744)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab270744 has not yet been referenced specifically in any publications.
