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RecombinantRabMAb

Recombinant Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Submit a review Submit a question References (3)
Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
  • Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5367-62] to Lambda Light chain
  • Suitable for: Flow Cyt (Intra), WB, ELISA, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free HRP

Overview

  • Product name

    Anti-Lambda Light chain antibody [EPR5367-62]
    See all Lambda Light chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5367-62] to Lambda Light chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ELISA, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Human Lambda Light chain. Purified Human IgA.

  • Positive control

    • WB: Human tonsil, plasma, thymus and spleen lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: Ramos cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5367-62

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/20000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/50000 - 1/200000.
ELISA
1/64000.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF
1/250.

For unpurified use at 1/500 - 1/1000.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/20000. Predicted molecular weight: 25 kDa.

For unpurified use at 1/50000 - 1/200000.
ELISA
1/64000.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF
1/250.

For unpurified use at 1/500 - 1/1000.

Target

  • Relevance

    All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • Bence Jones protein antibody
      • BJP antibody
      • Constant region of lambda light chains antibody
      • Ig lambda chain C regions antibody
      • ig lambda-6 chain C region antibody
      • IGLC 1 antibody
      • IGLC 2 antibody
      • IGLC 3 antibody
      • IGLC antibody
      • IGLC1 antibody
      • IGLC2 antibody
      • IGLC3 antibody
      • IGLC6 antibody
      • IGLV antibody
      • Immunoglobulin lambda constant 1 antibody
      • Immunoglobulin lambda constant regin 1 antibody
      • immunoglobulin lambda gene cluster antibody
      • Immunoglobulin lambda locus antibody
      • Immunoglobulin lambda variable cluster antibody
      • Immunoglobulin: lambda light chain antibody
      • Mcg marker antibody
      • Paraprotein antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Flow Cytometry (Intracellular) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

      Intracellular Flow Cytometry analysis of Ramos (human Burkitt's lymphoma) cells labeling Lambda Light chain with unpurified ab124719 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

       

       

    • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/100000 dilution (purified) + Human plasma lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/20000 dilution (purified) + Human thymus tissue lysate at 20 µg

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 25 kDa
      Observed band size: 25 kDa



      Blocking and dilution buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Lambda Light chain with purified ab124719 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Immunocytochemistry/ Immunofluorescence - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

      Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling Lambda Light chain with purified ab124719 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

      Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    • ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      ELISA - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

      ELISA analysis of Human Kappa light chain (Free), Human Lambda Light Chains (Free), Human IgA, Human IgM, Human IgG, Rat IgG, Mouse IgG at 1000 ng/mL with ab124719 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    • Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Western blot - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      All lanes : Anti-Lambda Light chain antibody [EPR5367-62] (ab124719) at 1/50000 dilution (unpurified)

      Lane 1 : Human tonsil lysate
      Lane 2 : Human plasma lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 25 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Lambda Light chain with unpurified ab124719 at a dilution of 1/250.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)
      Anti-Lambda Light chain antibody [EPR5367-62] (ab124719)

    References (3)

    Publishing research using ab124719? Please let us know so that we can cite the reference in this datasheet.

    ab124719 has been referenced in 3 publications.

    • Deng Z  et al. Expression of immunoglobulin G in human proximal tubular epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33760139
    • Jing Z  et al. Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion. Int J Mol Med 41:3296-3306 (2018). PubMed: 29512722
    • Zhang L  et al. Hsp70 inhibition induces myeloma cell death via the intracellular accumulation of immunoglobulin and the generation of proteotoxic stress. Cancer Lett 339:49-59 (2013). WB ; Human . PubMed: 23887058

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