Recombinant Anti-Lambda Light chain antibody [SP147] (ab227653)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP147] to Lambda Light chain
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Lambda Light chain antibody [SP147]
See all Lambda Light chain primary antibodies -
Description
Rabbit monoclonal [SP147] to Lambda Light chain -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human spleen lysate. IHC-P: Human tonsil, thyroid, B-cell lymphoma and follicule lymphoma tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP147 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
For antigen retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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WB |
1/100. Predicted molecular weight: 23 kDa.
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Notes |
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IHC-P
1/100. For antigen retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
WB
1/100. Predicted molecular weight: 23 kDa. |
Target
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Relevance
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 3535 Human
- Entrez Gene: 3546 Human
- Entrez Gene: 8217 Human
- Omim: 147220 Human
- SwissProt: P01701 Human
- SwissProt: P01842 Human
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Alternative names
- Bence Jones protein antibody
- BJP antibody
- Constant region of lambda light chains antibody
see all
Images
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Anti-Lambda Light chain antibody [SP147] (ab227653) at 1/100 dilution + Human spleen lysate
Predicted band size: 23 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [SP147] (ab227653)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [SP147] (ab227653)
Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [SP147] (ab227653)
Formalin-fixed, paraffin-embedded human follicule lymphoma tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lambda Light chain antibody [SP147] (ab227653)
Formalin-fixed, paraffin-embedded human thyroid tissue stained for Lambda Light chain using ab227653 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227653 has not yet been referenced specifically in any publications.